Phospho-p70 S6 Kinase Beta (Ser423) Rabbit Polyclonal Antibody#abs131111

abs131111-50ug

1-2 Weeks

201

abs131111-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Antibody for Phospho-p70 S6 Kinase beta (Ser423) is specifically designed to recognize and detect the presence of phosphorylated p70 S6 Kinase beta at Serine 423. It is highly sensitive in detecting the endogenous levels of this protein only when it is phosphorylated at this specific site. By specifically targeting phosphorylated p70 S6 Kinase beta, this antibody enables researchers to study the functional and regulatory mechanisms associated with this particular phosphorylation event. It is a valuable tool for investigating the role of p70 S6 Kinase beta in various cellular processes and signaling pathways.

Application

The recommended dilution for Western blotting is between 1:500 to 1:2000, while the ideal range for IF/ICC is 1:100 to 1:500. For ELISA assays using peptide, a dilution between 1:20000 to 1:40000 is recommended. These recommended dilutions ensure accurate and specific results while minimizing background noise. It is important to carefully optimize the dilution for each assay to ensure the best possible performance.

A peptide that has been synthesized from the human p70 S6 Kinase beta, which is situated around the phosphorylation site of Serine 423, is available. To create a highly similar content, the original text should be rearranged in a way that conveys the same information.

Properties

1mg/ml

Purification

The purified rabbit serum was used to obtain the antibody through a process of affinity purification involving sequential chromatography on phospho- and non-phospho-peptide affinity columns. Let me provide you with a highly similar content based on the original text information, but rearranged to ensure its uniqueness.

Clonality

Polyclonal Antibody

It is recommended to store the product at a temperature of -20 °C for a duration of one year. To maintain the product's quality, it should be protected from undergoing repeated freeze/thaw cycles.

Storage buffer

The Rabbit IgG solution comes in a phosphate buffered saline with a pH value of 7.4, containing 150mM NaCl and 0.02% sodium azide. The solution also contains 50% glycerol, making it viscous and easy to handle. To ensure stability, the solution should be stored at a temperature of -20 °C. It has a shelf life of 12 months from the date of receipt, allowing for long-term storage without any degradation in quality.

Target

Background

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Celluar localization

Nucleus;

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