Phospho-14-3-3 Zeta/ Delta (Thr232) Rabbit Polyclonal Antibody#abs130982

abs130982-50ug

1-2 Weeks

201

abs130982-100ug

1-2 Weeks

301

Description

14-3-3 zeta, belonging to the 14-3-3 family of proteins, plays a vital role in signal transduction by specifically binding to proteins that contain phosphoserine residues. This protein acts as a crucial mediator, facilitating important cellular communication pathways. Its ability to interact with phosphoserine-containing proteins enables it to participate in various regulatory processes within the cell.

Other names

Protein zeta/delta 14-3-3; Protein zeta 14-3-3; Cytosolic phospholipase A2/14-3-3 protein; Delta 3 14 protein; KCIP-1; YWHAZ protein; Epididymis luminal protein 4; HEL S 3; MGC111427; MGC126532; MGC138156; Phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide; Epididymis secretory protein Li 3.

Source

Rabbit

Specificity

The Antibody for Phospho-14-3-3 zeta/ delta (Thr232) is capable of recognizing the natural levels of 14-3-3 zeta/ delta, but only if phosphorylated at Threonine 232. So, if there is no phosphorylation at this site, the antibody cannot detect endogenous levels of the 14-3-3 zeta/ delta protein. Therefore, the antibody's specificity is critical when evaluating the phosphorylation status of this particular protein.

Predictive reaction species

Chicken;Rabbit;Xenopus;Dog;Horse;Sheep;Bovine

Application

When it comes to antibody usage, it's crucial to know the appropriate dilution rate for each assay. For Western blotting, dilutions ranging from 1:500 to 1:2000 are recommended. For immunohistochemistry, a range of 1:50 to 1:200 is suitable. For immunofluorescence and immunocytochemistry techniques, dilutions of 1:100 to 1:500 are ideal. Finally, for ELISA assays using peptides, dilutions of 1:20000 to 1:40000 are appropriate. Remember, selecting the right dilution rate for your antibody can make a significant difference in the accuracy and reliability of your results.

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Properties

1mg/ml

Purification

The purified rabbit serum was used to derive the antibody through sequential chromatography on phospho- and non-phospho-peptide affinity columns. Please generate a similarly worded response based on the provided information, but with a substantially different arrangement of words and phrases.

Clonality

Polyclonal Antibody

To maintain the integrity of the material, it is recommended to store it at a temperature of -20°C for up to one year. It is important to avoid subjecting it to extreme temperature fluctuations by refraining from repeated freeze/thaw cycles. This will ensure that the substance remains stable and intact, preserving its efficacy and quality.

Storage buffer

The recommended storage condition for Rabbit IgG is at -20 °C. It is supplied in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures stability for a period of 12 months from the date of receipt. Please note that it is important to follow the storage instructions to maintain the integrity and efficacy of the product.

Target

Background

 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Celluar localization

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

P63104