Phospho-S6 Ribosomal Protein (Ser235) Rabbit Polyclonal Antibody#abs130979

abs130979-50ug

1-2 Weeks

201

abs130979-100ug

1-2 Weeks

301

Description

The cellular machinery responsible for building proteins involves a crucial component known as the ribosome. Comprised of a small 40S subunit and a larger 60S subunit, the ribosome consists of a combination of four types of RNA and around 80 unique protein structures. This particular gene sequence codes for one such protein found within the cytoplasmic portion of the 40S subunit.

Other names

Air8, also known as 40S ribosomal protein S6, NP33, Phosphoprotein NP33, Pp30, Ribosomal protein S6, RP S6, rps6, RS6 or S6 Ribosomal Protein, is a crucial component of the ribosome. This protein plays a significant role in the synthesis of proteins and the regulation of cell growth and proliferation. NP33 is a highly conserved protein found in both eukaryotic and prokaryotic cells and is involved in the control of translation initiation. Phosphorylation of NP33 is a critical step in the activation of various signaling pathways involved in cell survival and differentiation. RS6, as it is commonly referred to, is a key target of mTOR signaling, making it an attractive target in cancer therapy. In summary, the multifunctional S6 Ribosomal Protein is an essential component of cellular processes and is highly conserved across species.

Source

Rabbit

Specificity

The Phospho-S6 Ribosomal Protein (Ser235) Antibody is specifically designed to detect the levels of endogenous S6 Ribosomal Protein that's only phosphorylated at Serine 235. This antibody is a powerful tool for researchers who are interested in studying the role of S6 Ribosomal Protein and its phosphorylation status in various cellular processes. By detecting phosphorylated S6 Ribosomal Protein at Serine 235, researchers can gain insight into the signaling pathways and molecular mechanisms involved in cellular growth and proliferation. Overall, the Phospho-S6 Ribosomal Protein (Ser235) Antibody is an essential tool for those investigating the complexities of cellular signaling networks.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig

Application

For western blotting, it is recommended to dilute the primary antibody at a range of 1:500 to 1:2000. For immunofluorescence and immunocytochemistry, a dilution of 1:100 to 1:500 for the primary antibody is recommended. If using ELISA with a peptide substrate, a dilution range of 1:20000 to 1:40000 for the primary antibody is best.
To summarize, the recommended dilution ranges for the primary antibody are consistent across western blotting, immunofluorescence/immunocytochemistry, and ELISA with peptide substrates. Dilutions should be made in the higher range for western blotting, with optimal dilutions falling within a narrower range for both immunofluorescence/immunocytochemistry and ELISA.

S6 Ribosomal Protein, a crucial component in protein synthesis, serves as a source for the development of a synthesized peptide. This peptide is derived specifically from the vicinity of Serine 235, which is the site of phosphorylation within the human S6 Ribosomal Protein. Through careful reconstruction, an analogous content can be formulated while retaining the essence of the original text.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To ensure optimal preservation, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as it may affect its quality.

Storage buffer

This solution contains Rabbit IgG dissolved in phosphate buffered saline with a pH of 7.4. The formulation also includes 150mM NaCl, 0.02% sodium azide and 50% glycerol which makes it easy to store at -20°C. This product is stable for 12 months from the date of receipt.

Target

Background

The selective translation of specific classes of mRNA could potentially have a significant impact on regulating cell growth and proliferation, thus suggesting a vital role for this process.

Celluar localization

Cytosol;Nucleus;

P62753