Phospho-Lamin A/C (Ser392) Rabbit Polyclonal Antibody#abs130684

abs130684-50ug

1-2 Weeks

201

abs130684-100ug

1-2 Weeks

301

Description

The inner nuclear membrane is flanked by a two-dimensional matrix of proteins known as the nuclear lamina. This matrix is primarily composed of the lamin family of proteins, which have remarkably high levels of conservation throughout evolution. One crucial aspect of the lamina matrix is its ability to undergo reversible disassembly during mitosis. This disassembly occurs when the lamin proteins undergo phosphorylation, leading to dynamic changes in the nuclear structure.

Other names

Lamin A/C, also known as LMNA, is a protein that plays an important role in various cellular processes, including DNA replication, transcription, and chromatin organization. It is encoded by the LMNA gene and has a molecular weight of 70 kDa.
Mutations in the LMNA gene have been associated with several diseases, including cardiomyopathy dilated 1A (autosomal dominant), limb girdle muscular dystrophy 1B (autosomal dominant), Charcot-Marie-Tooth disease type 2B1, Hutchinson-Gilford progeria syndrome, and idiopathic dilated cardiomyopathy.
In addition to its role in disease pathogenesis, lamin A/C has also been implicated in tumorigenesis. The protein is overexpressed in several types of cancer, including renal carcinoma, and has been proposed as a potential biomarker for diagnosis and prognosis.
Overall, lamin A/C is a multifunctional protein with diverse roles in normal and pathological cellular processes. Further research is needed to fully understand its functions and potential therapeutic applications.

Source

Rabbit

Specificity

The detection of Lamin A/C protein requires the presence of phosphorylation at Serine 392. This antibody specifically targets and recognizes endogenous levels of Lamin A/C that have undergone phosphorylation at this site. By analyzing the phosphorylation status at Serine 392, the antibody enables the identification of Lamin A/C protein in various biological samples. It is important to note that this antibody does not detect the non-phosphorylated form of Lamin A/C.

Predictive reaction species

Zebrafish;Pig;Rabbit;Horse;Bovine

Application

We recommend using the following dilutions for different applications: Western blot (WB) at 1:500-1:2000, immunohistochemistry (IHC) at 1:50-1:1000, immunofluorescence/immunocytochemistry (IF/ICC) at 1:100-1:500, and enzyme-linked immunosorbent assay (ELISA) using peptide at 1:20000-1:40000.

。，Lamin A/C392。

Properties

1mg/ml

Purification

The purification process of the antibody involves affinity purification using phospho- and non-phospho-peptide affinity columns on purified rabbit serum. By sequentially conducting chromatography on these columns, the antibody is obtained.

Clonality

Polyclonal Antibody

To ensure the preservation of the item, store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

The Rabbit IgG is stored in a solution containing phosphate buffered saline, with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at a temperature of -20°C, and can remain stable for up to 12 months from the date of receipt. It is important to note that any attempts to modify the contents of this storage solution may result in changes to the properties or effectiveness of the product. Therefore, it is recommended that this solution be used as is, without any alterations.

Target

Background

 Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.

Tissue specificity

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;

P02545