Phospho-Abl (Tyr204) Rabbit Polyclonal Antibody#abs130666

abs130666-50ug

1-2 Weeks

201

abs1306066-100ug

1-2 Weeks

301

Description

The ABL1 protooncogene is responsible for encoding a protein tyrosine kinase found in both the cytoplasm and nucleus. Its involvement has been discovered in various cellular processes including cell differentiation, division, adhesion, and response to stress. The c-Abl protein is normally kept in check by its SH3 domain, which acts as a negative regulator. Interestingly, when the SH3 domain is deleted, ABL1 transforms into an oncogene, potentially leading to the development of cancer.

Other names

Abelson murine leukemia viral oncogene homolog 1, also known as Abelson tyrosine protein kinase 1 or Abl 1, is a proto oncogene tyrosine protein kinase that plays a pivotal role in numerous cellular processes, including cell division, differentiation, adhesion, and migration. ABL1 encodes a non-receptor tyrosine kinase that functions in a variety of signal transduction pathways, including those that regulate gene expression, cell cycle progression, and apoptosis.
In addition, ABL1 is implicated in several human diseases, including chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The BCR/ABL fusion protein, which results from the translocation of chromosome 9 and 22, is the hallmark of CML and ALL. This fusion protein has constitutive tyrosine kinase activity, leading to the activation of downstream signaling pathways that promote cell proliferation and survival.
Despite its role in disease, ABL1 has also been shown to have important physiological functions. For instance, ABL1 is required for proper neural development and function, and its dysregulation has been implicated in neurodegenerative disorders such as Alzheimer's disease.
Overall, the diverse functions of ABL1 underscore its importance in cellular physiology and pathology.

Source

Rabbit

Specificity

The detection capability of the Phospho-Abl (Tyr204) Antibody is restricted to the phosphorylated form of Abl at Tyrosine 204. It specifically recognizes the endogenous levels of Abl protein only when it is phosphorylated at this specific tyrosine residue. Please note that this antibody will not detect Abl in its non-phosphorylated state or at other phosphorylation sites.

Predictive reaction species

Zebrafish;Chicken;Pig;Dog;Horse;Sheep;Bovine

Application

The recommended dilutions for Western blotting are 1:500-1:2000, while for immunofluorescence and immunocytochemistry the range is 1:100-1:500. For ELISA using peptide substrates, the suggested dilutions are 1:20000-1:40000. These dilutions have been carefully determined to ensure optimal signal detection while minimizing background noise. It is important to follow the recommended dilutions to achieve accurate and reliable results in your experiments.

A peptide synthesized from human Abl at the Tyrosine 204 phosphorylation site is now available. This peptide closely resembles the original human Abl, with a focus on the critical Tyrosine 204 residue. The peptide is highly similar in structure and function and may prove useful in a variety of research applications.

Properties

1mg/ml

Purification

This particular antibody was obtained through the purification of rabbit serum using affinity purification techniques. Sequential chromatography was conducted on both phospho- and non-phospho-peptide affinity columns to isolate and extract the desired antibody. The resulting antibody is highly purified and possesses properties similar to those of the original serum.

Clonality

Polyclonal Antibody

To ensure the quality of the product, it is recommended to store it at -20 °C for a duration of one year. It is vital to avoid subjecting the product to repeated freeze/thaw cycles, as this may have a negative impact on its stability. It is advised to follow these storage instructions diligently to maintain the integrity and effectiveness of the product.

Storage buffer

The storage instructions for Rabbit IgG state that it should be kept in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol, and stored at -20 °C. The product is stable for up to 12 months from the date of receipt. It's important to follow these guidelines to ensure the integrity and effectiveness of the product.

Target

Background

 Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Tissue specificity

Widely expressed.

Celluar localization

Cytoskeleton;Cytosol;Mitochondrion;Nucleus;Plasma Membrane;

P00519