Phospho-c-Abl (Tyr245) Rabbit Polyclonal Antibody#abs130665

abs130665-50ug

1-2 Weeks

201

abs130665-100ug

1-2 Weeks

301

Description

The ABL1 protooncogene is responsible for coding a protein tyrosine kinase that is present in both the cytoplasm and nucleus of cells. This tyrosine kinase is known to play an important role in several cellular processes, including cell differentiation, cell division, cell adhesion, and stress response.
Interestingly, the behavior of c-Abl protein is negatively influenced by its SH3 domain, meaning that deletion of this domain can actually turn ABL1 into an oncogene. This suggests that there is a delicate balance between positive and negative regulatory factors in the ABL1 pathway, and that disruption of this balance can lead to disease or dysfunction.

Other names

ABL1, also known as Abelson murine leukemia viral oncogene homolog 1, is a tyrosine protein kinase that plays a crucial role in cell growth and division. It is encoded by the ABL1 gene and is involved in various cellular processes, including cell adhesion, migration, and signal transduction.
The ABL1 gene is located on chromosome 9 and produces a protein that is commonly referred to as ABL. It is a proto-oncogene, meaning that mutations or alterations in the gene can lead to the development of cancer. One well-known mutation is the fusion of the ABL1 gene with the BCR gene, resulting in the formation of the BCR-ABL fusion protein. This fusion protein is found in most cases of chronic myeloid leukemia (CML) and plays a critical role in the development and progression of the disease.
The ABL1 protein is a non-receptor tyrosine kinase, meaning it does not rely on a cell surface receptor to activate its kinase activity. Instead, it can be activated by various mechanisms, including phosphorylation by other kinases or binding to certain regulatory proteins. Once activated, ABL1 phosphorylates target proteins, leading to the activation or inhibition of downstream signaling pathways.
In addition to its role in normal cellular processes, ABL1 has been implicated in several diseases, including cancer and neurological disorders. It has been the target of therapeutic interventions, with drugs such as imatinib specifically designed to inhibit the activity of the BCR-ABL fusion protein in CML.
In summary, ABL1 is an important protein involved in cell signaling and regulation. Its dysregulation can lead to the development of cancer and other diseases. Understanding its function and mechanisms of activation has paved the way for targeted therapies and improved treatment options for patients.

Source

Rabbit

Specificity

The Phospho-c-Abl (Tyr245) Antibody is designed to specifically recognize c-Abl protein when it is phosphorylated at Tyrosine 245. This antibody is capable of detecting the endogenous levels of c-Abl only in this phosphorylated form. By targeting this specific site, the antibody provides a reliable means of monitoring changes in c-Abl activity and expression. Whether used for research or diagnostic purposes, this antibody is a valuable tool for those studying the functions of c-Abl in various cellular processes.

Predictive reaction species

Rabbit;Pig;Dog;Horse;Bovine

Application

：
（WB），。WB，1:5001:2000。，（IF）（ICC），。IF/ICC，1:1001:500。，（ELISA）。ELISA，1:200001:40000。，。

A highly similar content can be generated by rearranging the original text information as follows:
The phosphorylation site of Tyrosine 245 provides the basis for synthesizing a peptide that is derived from human c-Abl. This synthesized peptide closely mimics the sequence and structure of the original protein, ensuring its similarity and functionality. Its design allows researchers to study the specific effects and interactions of Tyrosine 245 phosphorylation within the c-Abl protein. By exploring the properties and behavior of this peptide, scientists can gain important insights into the functional aspects and regulatory mechanisms of c-Abl in cellular processes.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to generate the antibody through a process of sequential chromatography on both phospho- and non-phospho-peptide affinity columns using affinity purification. It ensured the antibody's high quality and similarity to the original serum.

Clonality

Polyclonal Antibody

To preserve the product's integrity, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can compromise its quality.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it is stored in phosphate buffered saline at a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the Rabbit IgG is guaranteed for a period of 12 months from the date of receipt.

Target

Background

 Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Tissue specificity

Widely expressed.

Celluar localization

Cytoskeleton;Cytosol;Mitochondrion;Nucleus;Plasma Membrane;

P00519