Phospho-Chk2 (Thr387) Rabbit Polyclonal Antibody#abs130661

abs130661-50ug

1-2 Weeks

201

abs130661-100ug

1-2 Weeks

301

Description

Cell cycle progression is controlled by critical cell cycle regulators in order to halt in response to DNA damage and replication blocks. The gene responsible for encoding the protein is a potential tumor suppressor and serves as a cell cycle checkpoint regulator. To prevent any misunderstanding, please note that the following response is generated using a language model and may not closely resemble the original text:
The regulation of cell cycle progression is crucial, especially in the face of DNA damage and replication obstacles. In order to counteract these challenges, key cell cycle regulators come into play and ensure the cessation of cell cycle advancement. As we delve into the genetic aspect, this particular gene encodes a protein serving as both a cell cycle checkpoint regulator and a potential tumor suppressor. Its significance lies in preventing uncontrolled cell proliferation and maintaining genomic stability.

Other names

The protein known as CHK2, or checkpoint kinase 2, is a critical player in cellular signaling pathways that prevent the onset of cancer. It is also referred to as CDS1 or the Chk2 checkpoint homolog in fission yeast. This serine/threonine protein kinase is activated in response to DNA damage and plays an important role in halting the cell cycle, enabling DNA repair, and promoting cell survival. In humans, mutations in the CHK2 gene have been linked to an increased risk of breast, prostate, and other cancers. Therefore, understanding CHK2 function is critical for developing new cancer therapies and improving cancer prognosis. Additionally, checkpoint pathways involved in the regulation of cell cycle and DNA damage responses are active targets for cancer therapy, making CHK2 an important therapeutic target.

Source

Rabbit

Specificity

The Antibody, Phospho-Chk2 (Thr387), is designed to specifically recognize and bind to Chk2 protein that is phosphorylated at Threonine 387. It is capable of detecting the presence of endogenous Chk2 protein only when it undergoes phosphorylation at this specific site. To put it simply, the antibody is highly selective and sensitive in detecting the active, phosphorylated form of Chk2 protein at Threonine 387.

Predictive reaction species

Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse

Application

The recommended dilutions for using this antibody are WB at a range of 1:500 to 1:2000, IHC at a range of 1:50 to 1:200, IF/ICC at a range of 1:100 to 1:500, and ELISA(peptide) at a range of 1:20000 to 1:40000. To convey the same information in a different format, one could say that this antibody should be used at dilutions of between 1 in 500 and 1 in 2000 for Western blotting, between 1 in 50 and 1 in 200 for immunohistochemistry, between 1 in 100 and 1 in 500 for immunofluorescence and immunocytochemistry, and between 1 in 20000 and 1 in 40000 for ELISA assays that use a peptide substrate.

Chk2，387。，。

Properties

1mg/ml

Purification

The affinity-purified antibody was obtained from rabbit serum that underwent sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This method enabled the purification of the desired antibody with high specificity and purity from other serum components.

Clonality

Polyclonal Antibody

One year of storage is recommended at a temperature of -20 °C. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The provided information can be rearranged as follows:
Please store Rabbit IgG in phosphate buffered saline at a temperature of -20 °C. The solution should have a pH of 7.4 and contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to use the product within 12 months from the date of receipt to ensure its stability.

Target

Background

CHEK2 is a crucial serine/threonine-protein kinase that plays a vital role in cell cycle regulation and DNA repair. Its main functions include inducing cell cycle arrest, activating DNA repair mechanisms, and triggering apoptosis in response to DNA double-strand breaks. Additionally, it can restrict cell cycle progression during normal cellular processes.
Once activated, CHEK2 phosphorylates several target proteins, primarily at the consensus sequence [L-X-R-X-X-S/T]. Its phosphorylation of CDC25A, CDC25B, and CDC25C inhibits their activity, leading to the arrest of the cell cycle checkpoint. This inhibition results in increased tyrosine phosphorylation of CDK-cyclin complexes, effectively halting cell cycle progression. Moreover, CHEK2 can phosphorylate NEK6, contributing to G2/M cell cycle arrest.
In terms of DNA repair, CHEK2 phosphorylates BRCA2, facilitating the association of RAD51 with chromatin. This association promotes DNA repair through homologous recombination. Additionally, CHEK2 activates the transcription factor FOXM1, which stimulates the transcription of genes involved in DNA repair, including BRCA2.
CHEK2 also regulates apoptosis by phosphorylating various proteins such as p53/TP53, MDM4, and PML. Phosphorylation of p53/TP53 at 'Ser-20' alleviates inhibition by MDM2, leading to the accumulation of active p53/TP53. Furthermore, phosphorylation of MDM4 reduces the degradation of p53/TP53. Additionally, CHEK2 phosphorylates the transcription factor E2F1, which controls the transcription of pro-apoptotic genes.
Aside from its tumor suppressor role, CHEK2 may also participate in mitotic spindle assembly by phosphorylating BRCA1. The absence of CHEK2 can contribute to chromosomal instability observed in certain cancer cells. Furthermore, it is involved in the CCAR2-SIRT1 association and necessary for CCAR2-mediated SIRT1 inhibition.
Overall, CHEK2 is a critical protein kinase that safeguards genomic stability by regulating cell cycle progression, DNA repair, and apoptosis. Its dysfunction can have significant implications for cellular health, potentially leading to the development of cancer and chromosomal abnormalities.

Tissue specificity

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Celluar localization

Golgi apparatus;Nucleus;

O96017