Phospho-SNAI1 (Ser246) Rabbit Polyclonal Antibody#abs130660

abs130660-50ug

1-2 Weeks

201

abs130660-100ug

1-2 Weeks

301

Description

During postimplantation development, this protein plays multiple crucial roles. It not only participates in the formation and preservation of embryonic mesoderm but also potentially contributes to chondrogenesis and epithelial-mesenchymal inductive interactions. Its involvement spans various aspects of development and demonstrates its significance in these processes.

Other names

The protein SNA is also known as Snail homolog 1 (SNAI1) or Snail 1 zinc finger protein. It is encoded by the SNAIL1 gene and is a transcriptional repressor that plays a crucial role in embryonic development, epithelial-mesenchymal transition, and tumor progression. SNA is homologous to the Snail protein found in Drosophila, hence the name Snail homolog 1 (Drosophila). Other names for SNA include SLUGH2, SNAI, snai1, SNAI1_HUMAN, Snail 1 homolog, and Zinc finger protein SNAI1. Its function is to regulate cell adhesion, migration, and differentiation by directly targeting E-cadherin, a cell-cell adhesion protein. SNA also interacts with other transcription factors, such as Twist and Slug, to regulate gene expression. Its overexpression has been implicated in multiple types of cancers, making it a potential target for cancer therapy.

Source

Rabbit

Specificity

The Phospho-SNAI1 (Ser246) Antibody specifically recognizes the phosphorylated Serine 246 residue on endogenous levels of SNAI1. It does not detect non-phosphorylated SNAI1.

Predictive reaction species

Rabbit;Horse;

Application

The recommended dilution for Western blot is 1:500-1:2000, for Immunofluorescence/Immunocytochemistry is 1:100-1:500, and for Enzyme-Linked Immunosorbent Assay using peptide is 1:20000-1:40000. You can obtain similar information by rearranging the text in different ways.

A synthesized peptide has been developed from the human SNAI1 protein, specifically targeting the phosphorylation site of Serine 246. The composition of the peptide closely resembles that of the original protein, ensuring its effectiveness in biological systems. This development marks an important step in understanding the function of the SNAI1 protein and its role in various pathological conditions. The use of synthetic peptides in research and clinical applications has proven to be a valuable tool for investigating the cell signaling pathways, protein-protein interactions and drug discovery. The synthesized peptide is expected to have potential applications in the development of novel therapies for various diseases.

Properties

1mg/ml

Purification

The purified rabbit serum was used to obtain the antibody through a process of sequential chromatography on affinity columns for phospho- and non-phospho-peptides. The resultant antibody is highly specific and of superior quality.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is advised to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles.

Storage buffer

The storage condition for Rabbit IgG is as follows: it should be stored at a temperature of -20 °C in phosphate buffered saline with a pH of 7.4. Additionally, it should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product remains stable for a period of 12 months starting from the date of receipt.

Target

Background

The protein in question plays a pivotal role in many fundamental cellular processes such as embryonic mesoderm formation, induction of the epithelial to mesenchymal transition (EMT), cell migration, survival, and growth arrest. It binds to various gene promoters including the E-cadherin/CDH1 promoter, where it associates with histone demethylase, KDM1A, to initiate transcriptional repression and a decrease in dimethylated H3K4 levels. Additionally, it forms complexes with EGR1 and SP1 to up-regulate CDKN2B in response to tetradecanoyl phorbol acetate (TPA) exposure. It may also act as an individual activator of the CDKN2B promoter. In summary, the protein has a diverse array of functions in cellular processes such as gene transcription, cell migration, and growth control.

Tissue specificity

Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.

Celluar localization

Nucleus;

O95863