Rabbit Anti-Endonuclease G Polyclonal Antibody #abs124466

abs124466-50ul

In Stock

201

abs124466-100ul

In Stock

301

Other names

EndoG, also known as Endonuclease G, is a mitochondrial protein. It is encoded by the FLJ27463 gene in humans and is commonly referred to as ENDOG or NUCG_HUMAN. EndoG plays a critical role in DNA degradation during apoptosis. Its function involves cleaving DNA at specific sites, ultimately leading to the fragmentation of genomic DNA. This enzyme has been extensively studied and is believed to contribute to the efficient removal of nuclear DNA during the late stages of apoptosis. The importance of EndoG in cellular processes makes it an intriguing target for further research and potential therapeutic applications.

Source

Rabbit

Specificity

Endo G

Species Reactivity

As we look at the list of animal species - human, mouse, rat, dog, pig, cow, horse, rabbit, sheep, and guinea pig - it's clear that these creatures couldn't be more different from one another. However, whether we are examining the genetic makeup or the behavior of these animals, commonalities can be found and observed. For example, many of these creatures possess unique physical and cognitive abilities that make them well-suited for a variety of tasks and roles in society. Additionally, each animal has its own unique place in the ecosystem, and plays a critical role in maintaining balance and harmony in the natural world.

Application

The working dilutions for WB, ELISA, IHC-P, IHC-F, and IF are as follows: WB should be diluted at 1:500-2000, ELISA at 1:500-1000, IHC-P at 1:400-800, IHC-F at 1:400-800, and IF at 1:100-500. It is important to note that antigen retrieval is required for paraffin section in IF.

Immunogen

Human Endo G:101-200/297 has been used to create a synthetic peptide which has been conjugated with KLH. This highly specific conjugate can be utilized for various biological assays and experiments. The sequence of this synthetic peptide is very identical to the original human Endo G protein fragment, ensuring reliable and accurate results. By using this conjugate, scientists can effectively study the mechanisms and functions of this important protein.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To maintain the quality of the product, it is important to store it at a temperature of -20 °C for up to a year. Keep in mind to prevent multiple freeze/thaw cycles as this can affect the integrity of the product.

Storage buffer

pH7.4，1%BSA、0.03%Proclin30050%0.01MTBS。，。

Research area

Cell biology of tumors, immunology of cancer and the role of chromatin and nuclear signaling pathways in cancer progression have been the focus of cancer research for decades. But, in recent years, there has been increasing interest in understanding the role of mitochondria in cancer development and therapy.
Studies have shown that the mitochondrial genome is frequently altered in cancer cells, and these changes can contribute to the metabolic reprogramming that characterizes cancer cells. Mitochondria are also involved in the regulation of cellular signaling pathways, including those that control cell proliferation and apoptosis.
In addition, recent work has identified a critical role for mitochondria in the regulation of the immune response to cancer. Mitochondrial dysfunction can impair the ability of immune cells to recognize and eliminate tumor cells, while alterations in mitochondrial metabolism can enhance the immunosuppressive properties of the tumor microenvironment.
Overall, a better understanding of the complex interplay between tumor cell biology, immunology, chromatin and nuclear signaling, and mitochondrial function will be essential for the development of more effective cancer therapies.

Target

Background

This gene encodes a nuclear endonuclease which is found inside the mitochondria and is responsible for cleaving DNA at GC tracts. Present in a wide range of animals, this protein plays a crucial role in the initiation of replication of mitochondrial DNA by generating RNA primers required by DNA polymerase gamma. The importance of this protein cannot be overstated as it helps in maintaining the integrity of mitochondrial DNA which is important for mitochondrial function and cellular metabolism.

Celluar localization

Nucleus cytoplasm mitochondria

UniPort

Q14249