FACL6 Antibody (Center) #abs106076

abs106076-50ul

In Stock

228

Description

Purified Rabbit Polyclonal Antibody (Pab)

Other names

There are several names used to refer to the same protein, including Long-chain-fatty-acid--CoA ligase 6, Long-chain acyl-CoA synthetase 6, LACS6, ACSL6, ACS2, FACL6, KIAA0837, and LACS5. Regardless of the name, this protein is essential in the process of activating long-chain fatty acids for their metabolic use. The ACSL6 enzyme is crucial in the process of lipid metabolism and is involved in transforming long-chain fatty acids into acyl-CoA esters. This catalytic protein is present in many tissues, including the liver, skeletal muscle, and adipose tissue. Its dysregulation has been found to be linked to numerous metabolic diseases, including obesity, diabetes, and cardiovascular diseases.

Source

Rabbit

Specificity

The FACL6 antibody is obtained from rabbits that were immunized with a synthetic peptide conjugated to KLH. This peptide corresponds to amino acids 240-270 in the central region of human FACL6. To create a highly similar content, you can rearrange the given information as follows:
Rabbits were immunized with a KLH-conjugated synthetic peptide derived from the central region of human FACL6. This peptide encompasses amino acids 240-270. The resulting antibody is a product of this immunization process.

Species Reactivity

Human;Mouse

Application

WB~~1:1000
IHC-P~~1:50~100

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To ensure optimal freshness, it is recommended to keep this product refrigerated between 2-8°C for a maximum of two weeks. For extended storage, it is advised to store the product in small aliquots and freeze at -20°C to prevent the occurrence of freeze-thaw cycles. This will help to maintain the integrity and quality of the product over the long term.

Storage buffer

The antibody provided is a purified polyclonal antibody that is accompanied by a phosphate-buffered saline (PBS) solution containing 0.09% (w/v) sodium azide. To obtain this purified antibody, a protein G column was used for purification. The antibody was eluted using buffers with high and low pH and was immediately neutralized. Following the purification process, the antibody was subjected to dialysis against PBS to further enhance its purity.

Research area

Cardiovascular;Metabolism;Signal Transduction

Target

Background

The activation of long-chain fatty acids serves a crucial role in brain metabolism. It is responsible for both synthesizing cellular lipids and enabling their degradation through beta-oxidation. Specifically, the acyl-CoAs produced during this process are exclusively utilized for the synthesis of brain lipids. This pivotal function ensures efficient fatty acid metabolism in the brain, supporting its essential functions.

Celluar localization

This protein is a single-pass type III membrane protein found in several cellular membranes. It can be found in the outer membrane of the mitochondrion, peroxisome membrane, microsome membrane, and endoplasmic reticulum membrane. Its function is currently unknown, but its presence in these membranes suggests a potential role in membrane stability or transport. Despite being found in multiple membranes, the protein's structure and function remain somewhat of a mystery and require further investigation.

UniPort

Q9UKU0

References

References

Yagasaki, F., et al., Genes Chromosomes Cancer 26(3):192-202 (1999).
Nagase, T., et al., DNA Res. 5(6):355-364 (1998).
Malhotra, K.T., et al., Biochem. J. 344 Pt 1, 135-143 (1999).