Rabbit Anti-ITPK1 Polyclonal Antibody #abs130440

abs130440-50ug

1-2 Weeks

201

abs130440-100ug

1-2 Weeks

301

Description

The ITPK1 kinase is a versatile enzyme that can phosphorylate different inositol polyphosphates, such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. One of its key functions is the ability to phosphorylate Ins(3,4,5,6)P4 at position 1, thereby forming Ins(1,3,4,5,6)P5. This process is significant because Ins(3,4,5,6)P4 inhibits plasma membrane Ca(2+)-activated Cl(-) channels, whereas Ins(1,3,4,5,6)P5 has no inhibitory effect.
In addition, ITPK1 can also phosphorylate Ins(1,3,4)P3 on O-5 and O-6, leading to the formation of Ins(1,3,4,6)P4. This molecule is crucial in the hexakisphosphate (InsP6) pathway and plays a vital role in numerous biological processes.
Moreover, ITPK1 acts as an inositol polyphosphate phosphatase that dephosphorylates Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3. Similarly, it can also dephosphorylate Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. ITPK1 is also an isomerase that can interconvert the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium.
Overall, ITPK1 is a crucial enzyme that catalyzes numerous reactions involving inositol polyphosphates. Its ability to phosphorylate, dephosphorylate, and isomerize these molecules makes it a critical player in various biological pathways.

Other names

The enzyme Inositol 1,3,4-trisphosphate (Ins(1,3,4)P3) 5/6-kinase, also known as ITPK1 or ITRPK1, plays a crucial role in cellular signaling. Its main function is to phosphorylate inositol 1,3,4-trisphosphate (Ins(1,3,4)P3) to generate inositol tetrakisphosphate 1 (Ins(1,3,4,5)P4). This phosphorylation reaction is essential for the regulation of various cellular processes.
ITPK1 is involved in the inositol phosphate metabolic pathway and specifically catalyzes the addition of phosphate groups to inositol 1,3,4-trisphosphate. This process leads to the synthesis of inositol tetrakisphosphate 1, which acts as a second messenger in intracellular signaling.
The importance of ITPK1 is highlighted by its involvement in numerous physiological processes. It has been implicated in the regulation of calcium release from intracellular stores, cell growth, and differentiation. Dysfunction or dysregulation of ITPK1 has been associated with various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
In summary, Inositol 1,3,4-trisphosphate 5/6-kinase, also known as ITPK1, is a crucial enzyme responsible for phosphorylating inositol 1,3,4-trisphosphate to produce inositol tetrakisphosphate 1. Its role in cellular signaling and its involvement in various physiological processes make it a highly significant protein in biological systems.

Source

Rabbit

Specificity

ITPK1 Antibody detects endogenous levels of ITPK1

Species Reactivity

Human;Mouse

Application

For western blotting, the recommended dilution range is 1:500-1:2000. When it comes to immunofluorescence and immunocytochemistry, a dilution range of 1:100-1:500 is suggested. For ELISA using a peptide substrate, the dilution range recommended is between 1:20000-1:40000. Please note that these are just guidelines and that optimal dilutions may vary depending on the specific experiment and conditions used.

Immunogen

A synthesized peptide derived from human ITPK1

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality, keep the store temperature at -20 °C for a duration of one year. Be careful not to subject the product to multiple cycles of freezing and thawing.

Storage buffer

This product contains Rabbit IgG in a phosphate buffered saline solution with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol as stabilizers. To maintain its quality, it should be stored at -20 °C. It can be kept in this condition for up to 12 months from the date of receipt before any noticeable degradation occurs. Feel free to ask any further questions regarding this information.

Target

Background

This enzyme exhibits a broad substrate specificity and is capable of phosphorylating various inositol polyphosphates such as Ins(3,4,5,6)P4 and Ins(1,3,4)P3. Its function in phosphorylating Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5 is of regulatory importance since Ins(3,4,5,6)P4 inhibits plasma membrane Ca2+-activated Cl- channels, whereas Ins(1,3,4,5,6)P5 does not possess inhibitory properties. Additionally, it phosphorylates Ins(1,3,4)P3 at positions O-5 and O-6, generating Ins(1,3,4,6)P4, which is essential for the hexakisphosphate (InsP6) pathway. Moreover, it functions as an inositol polyphosphate phosphatase, dephosphorylating Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. In the presence of ADP and magnesium, it also acts as an isomerase, interconverting the isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4. The enzyme likely serves as the limiting factor in the InsP6 pathway. It has been found to modulate TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.

Tissue specificity

Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.

Posttranslational modification

Acetylation by EP300 and CREBBP destabilizes ITPK1, and down-regulates enzymatic activity. Deacetylated by SIRT1.

Celluar localization

Cytosol;Plasma Membrane;

UniPort

Q13572