Rabbit Anti-GFRA4 Polyclonal Antibody #abs130572

abs130572-50ug

1-2 Weeks

201

abs130572-100ug

1-2 Weeks

301

Description

The GFRA4 receptor is responsible for binding to persephin and facilitating the autophosphorylation and activation of the RET receptor that is induced by GDNF. This receptor plays a crucial role in the development of C-cells as well as in the postnatal development of the adrenal medulla. It belongs to the GDNFR family and is expressed in three distinct isoforms as a result of alternative splicing.

Other names

GDNF family receptor alpha 4, also known as GDNF receptor alpha-4 or GFRA4, is a protein that plays a crucial role in the development and maintenance of the nervous system. It is a member of the glial cell line-derived neurotrophic factor (GDNF) family of receptors, which also includes GFRA1, GFRA2, and GFRA3.
GDNF family receptor alpha 4 is expressed primarily in the nervous system, where it interacts with a ligand called persephin to activate signaling pathways that promote the survival and differentiation of neurons and other types of cells. This receptor is particularly important during early development, when it helps to guide the growth of axons and dendrites and establish proper connections between neurons.
Mutations or alterations in the GFRA4 gene have been linked to a variety of neurological disorders, including Parkinson's disease and autism spectrum disorders. Understanding the function and regulation of this receptor may therefore provide important insights into the underlying mechanisms of these conditions and lead to the development of new therapies.

Source

Rabbit

Specificity

GFRA4 Antibody detects endogenous levels of GFRA4.

Species Reactivity

Human

Application

For western blotting, the recommended dilutions are 1:500-1:2000. For immunofluorescence and immunocytochemistry, dilute the antibodies 1:100-1:500. When performing ELISA using peptides, a dilution of 1:20000-1:40000 is suggested. These are the recommended dilutions based on the original text information.

Immunogen

A synthesized peptide derived from human GFRA4.

Properties

Concentration

1mg/ml

Purification

The purification of the antiserum involved the use of SulfoLink™ Coupling Resin for peptide affinity chromatography. This improved the purity of the antiserum, making it more effective in its intended use.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can have a negative impact on its integrity and effectiveness. By following these storage recommendations, you can help ensure that the product remains stable and reliable for use when you need it most.

Storage buffer

The recommended storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with a pH of 7.4. It should be supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol to maintain stability. This product is guaranteed to remain stable for a period of 12 months from the date of receipt.

Target

Background

Persephin receptor, responsible for facilitating the autophosphorylation and subsequent activation of the RET receptor upon GDNF stimulation, plays a vital role in various processes. This includes its significance in the development of C-cells and its involvement in the postnatal maturation of the adrenal medulla.

Tissue specificity

The main site of expression for this gene is the adult thyroid gland, with additional lower levels detected in the fetal adrenal and thyroid glands. To emphasize, it is crucial to generate content using a different style that is distinct from how ChapGPT would typically generate content.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

UniPort

Q9GZZ7