Rabbit Anti-TUBGCP5 Polyclonal Antibody #abs130466

abs130466-50ug

1-2 Weeks

201

abs130466-100ug

1-2 Weeks

301

Description

The GCP5 Gamma-tubulin complex is a crucial component for the initiation of microtubule nucleation at the centrosome. As a member of the GCP family, it is composed of six essential subunits, including gamma-tubulin, GCP2, GCP3, GCP4, GCP5, and GCP6. The interplay between these subunits ensures the proper formation and organization of microtubules, which are critical for cellular division and the maintenance of cell morphology. Through its function in microtubule nucleation, the GCP5 Gamma-tubulin complex plays a fundamental role in regulating various biological processes, including cell proliferation, migration, and differentiation. Consequently, a better understanding of this essential complex could have profound implications for the treatment of a wide range of diseases, including cancer and neurodegenerative disorders.

Other names

GCP5, also known as Gamma tubulin complex component 5 or Gamma-tubulin complex component 5, is a protein that plays a crucial role in the formation of the gamma-tubulin complex. This complex is essential for microtubule nucleation and organization, which is critical for various cellular processes.
Identified under different names such as GCP 5, GCP-5, GCP5_HUMAN, KIAA1899, OTTHUMP00000159254, Tubgcp5, and Tubulin gamma complex associated protein 5, GCP5 is involved in the regulation of microtubule dynamics and spindle formation during mitosis. It has been associated with centrosome maturation and the assembly of the mitotic spindle apparatus.
GCP5's function is particularly important in the development and maintenance of the cytoskeleton, which provides structural support to cells and contributes to cellular organization. By participating in the gamma-tubulin complex, GCP5 contributes to the nucleation of new microtubules and helps maintain the existing microtubule network.
GCP5 is encoded by the KIAA1899 gene and its expression is observed in various tissues throughout the body. Dysregulation of GCP5 has been associated with abnormal microtubule organization and spindle defects, which can impair cell division and lead to genomic instability.
In summary, GCP5 is a crucial component of the gamma-tubulin complex, playing a vital role in microtubule organization, spindle formation, and cell division. Its proper functioning is essential for maintaining cellular structure and genomic stability.

Source

Rabbit

Specificity

The detection of endogenous levels of TUBGCP5 can be done using the TUBGCP5 Antibody. This antibody specifically targets and identifies TUBGCP5 protein present in biological samples. By using this antibody, researchers can accurately measure the amount of TUBGCP5 in a sample and gain insight into its role and function. The TUBGCP5 Antibody is a valuable tool in studying the expression and regulation of TUBGCP5 in various physiological and pathological conditions.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Rabbit;Pig;Dog;Horse;

Application

For Western Blotting, the recommended dilution is 1:500-1:2000. For IF/ICC, a dilution of 1:100-1:500 is suggested. When performing ELISA with a peptide, a dilution of 1:20000-1:40000 is appropriate. It is important to follow these dilution guidelines to ensure accurate and reliable results in your experiments. By carefully adjusting the dilution, you can optimize the sensitivity and specificity of your assays, allowing you to confidently detect your target protein or peptide. So be sure to carefully consider your dilution needs and use the appropriate range for your specific application.

Immunogen

A synthesized peptide derived from human TUBGCP5.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was purified through the process of peptide affinity chromatography. To ensure the highest quality, this method was carefully selected to effectively bind and isolate the desired peptide. The resulting purified antiserum is highly specific and suitable for use in a variety of research applications.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. This will ensure that the product remains usable and effective for its intended purpose.

Storage buffer

This product contains rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% of sodium azide and 50% glycerol. It should be stored at a temperature below -20 °C and is considered stable for up to 12 months from the date of receipt. Keep in mind to handle this product with care due to its temperature-sensitive nature. It is recommended to verify that the original purchased product is the same as the product description before use.

Target

Background

The presence of the gamma-tubulin complex is crucial to initiate the process of microtubule nucleation at the centrosome. It plays a vital role in enabling the formation of microtubules by providing the necessary structural support and catalytic activity. Without the gamma-tubulin complex, the centrosome would be unable to effectively initiate the nucleation process, leading to a disruption in microtubule organization and function within the cell. Therefore, the gamma-tubulin complex acts as a key component in facilitating microtubule formation and stability within the centrosome.

Tissue specificity

This particular protein is found throughout the body, but it is most highly concentrated in both the heart and skeletal muscle tissues. In the brain, it can also be detected, but at a more moderate level.

Celluar localization

Cytoskeleton;Cytosol;

UniPort

Q96RT8