Rabbit Anti-Syndecan4 Polyclonal Antibody #abs130582

abs130582-50ug

1-2 Weeks

201

abs130582-100ug

1-2 Weeks

301

Description

The heparan sulfate proteoglycan encoded by this gene acts as a transmembrane receptor for intracellular signaling. Classified as a type I protein, it forms a homodimer and is a member of the syndecan proteoglycan family. This gene is located on chromosome 20, with a related pseudogene present on chromosome 22. Additional details can be found in SwissProt.

Other names

Syndecan 4, also known as amphiglycan or Ryudocan, is a proteoglycan that plays a crucial role in various cellular processes such as cell adhesion, migration, proliferation, and differentiation. The protein is encoded by the MGC22217 gene and also referred to as OTTHUMP00000031788. Additionally, it goes by the aliases Ryudocan amphiglycan and Ryudocan core protein.
Syndecan 4 belongs to the syndecan family of transmembrane heparan sulfate proteoglycans and is primarily expressed in epithelial, endothelial, and smooth muscle cells. It contains a conserved cytoplasmic domain, a transmembrane domain, and an extracellular domain composed of heparan sulfate chains and core protein.
Several functions have been linked to Syndecan 4, including serving as a receptor for extracellular matrix proteins, growth factors, and cytokines. Moreover, it regulates signaling pathways by interacting with various proteins and can modulate the actin cytoskeleton. Importantly, disruptions in Syndecan 4 expression and function have been implicated in several diseases, including cancer and cardiovascular diseases.
In summary, Syndecan 4 is a multifaceted molecule that plays a critical role in diverse cellular processes. Its significance in various diseases underscores the need to understand its function and regulation fully.

Source

Rabbit

Specificity

The detection of endogenous levels of Syndecan4 is accomplished by using the Syndecan4 Antibody. The generated content will be highly similar to the original text by rearranging the information while ensuring it remains based on the same context.

Species Reactivity

Human;Mouse;Rat

Application

IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000 are the recommended dilution ranges for various laboratory techniques. These dilutions are crucial for achieving optimal results when conducting immunohistochemistry (IHC), Western blot (WB), immunofluorescence (IF) or immunocytochemistry (ICC), and enzyme-linked immunosorbent assay (ELISA) using peptide antigens. It is important to carefully follow these dilution guidelines to ensure accurate and reliable experimental outcomes.

Immunogen

A synthesized peptide derived from human Syndecan4

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal preservation, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

This Rabbit IgG comes in a solution of phosphate buffered saline at a pH of 7.4 with 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store it at -20°C and it is stable for up to 12 months from the date of receipt.

Target

Background

A proteoglycan present on the cell surface, with heparan sulfate as its constituent molecule, has the ability to control exosome biogenesis in collaboration with SDCBP and PDCD6IP as per findings published on PubMed.

Tissue specificity

Expressed in epithelial and fibroblastic cells.

Posttranslational modification

Various factors, including heparanase, thrombin, and EGF, as well as stress and wound healing, contribute to the enhancement of shedding. Additionally, TIMP3 has been found to inhibit PMA-mediated shedding.

Celluar localization

Extracellular region or secreted;Golgi apparatus;Lysosome;Plasma Membrane;

UniPort

P31431