Phospho-EPHA2/3(Tyr588/596) Rabbit Polyclonal Antibody #abs130006

abs130006-50ug

1-2 Weeks

201

abs130006-100ug

1-2 Weeks

301

Description

EphA2 is a receptor tyrosine kinase that belongs to the Eph receptor tyrosine kinase family, which consists of 14 members. It binds to ephrin-A1, -A3, -A4, and -A5, which are membrane-anchored ligands involved in regulating the repulsion and adhesion of cells. This ultimately helps in establishing, maintaining, and remodeling patterns of cellular organization.
Eph signals play a crucial role in controlling cell adhesion and cell migration during development, axon guidance, homeostasis, and disease. The ephrin-Eph signaling system regulates various cellular processes, such as cell proliferation, differentiation, and survival.
These receptors have emerged as important targets for therapeutic interventions for various human diseases, including cancer, inflammatory disorders, and nervous system-related disorders. The Eph receptors have shown potential as promising targets for drug development due to their crucial role in regulating cell-cell interactions. Further research and development in this area hold significant promise for the treatment of various diseases.

Other names

EPHA2, also known as Ephrin receptor and Ephrin type A receptor 2, is a tyrosine protein kinase receptor that plays a crucial role in cell adhesion and migration. This receptor is encoded by the ARCC2, AW545284, CTPA, CTPP1, and CTRCT6 genes and is classified as EC 2.7.10.1.
Another important receptor is EPHA3, also known as Eph receptor A3 and Ephrin receptor EphA3. Encoded by the AW492086 gene, this receptor is an Eph-like tyrosine kinase 1 and also classified as EC 2.7.10.1. It is involved in the regulation of cell growth, differentiation, and angiogenesis.
Finally, EPHA4 is an Ephrin type-A receptor 4 that plays a role in axon guidance and the development of the nervous system. Encoded by the Cek 8 gene, this receptor is also known as EPH-like kinase 8 and is involved in cell adhesion and migration. Additionally, it has been linked to various neurological disorders.
Overall, these three tyrosine protein kinase receptors play important roles in various cellular processes and can serve as potential therapeutic targets for the treatment of diseases.

Source

Rabbit

Specificity

The Phospho-EPHA2/3/4(Tyr588/596) Antibody is designed to specifically detect endogenous levels of EPHA2 and EPHA3 only when they are phosphorylated at Tyrosine 588 and 596. This antibody enables researchers to study the activation and signaling of these proteins in various biological contexts. By targeting the specific phosphorylation sites, it allows for the characterization and analysis of EPHA2 and EPHA3 in their activated state.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse

Application

，：
。（WB），1:5001:2000。/（IF/ICC），1:1001:500。（ELISA），1:200001:40000。。，。

Immunogen

A synthesized peptide derived from human EPHA2/3/4 around the phosphorylation site of Tyrosine 588 and 596.

Properties

Concentration

1mg/ml

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Tissue specificity

Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

Posttranslational modification

Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB; serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated at Ser-897 in response to TNF by RPS6KA1 and RPS6KA3; RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). Phosphorylated at Ser-897 by PKA; blocks cell retraction induced by EPHA2 kinase activity (PubMed:27385333). Dephosphorylated by ACP1.Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).

Celluar localization

Plasma Membrane;

UniPort

P29317/P29320/P54764