Rabbit Anti-RCBTB1 Polyclonal Antibody #abs130184

abs130184-50ug

1-2 Weeks

201

abs130184-100ug

1-2 Weeks

301

Description

It is possible that RCBTB1 plays a role in regulating the cell cycle through chromatin remodeling mechanisms. This human protein has two distinct isoforms that are generated through alternative splicing. By reorganizing the information presented, it becomes apparent that this protein is likely involved in important cellular processes related to the regulation of gene expression and DNA structure. Through its actions on chromatin, RCBTB1 may help to ensure proper cell growth and division, which is essential for overall health and well-being.

Other names

CLL deletion region gene 7 protein, also known as CLLL7 or E4.5, is a protein encoded by the Rcbtb1 gene. It is an important regulator of chromosome condensation and belongs to the RCC1 and BTB domain-containing protein family. Its function as a guanine nucleotide exchange factor (GEF) like protein makes it a key player in the GDP/GTP exchange cycle. The hypothetical protein FLJ39335 is a highly similar protein to CLL deletion region gene 7 protein. Additionally, the gene is also known as RCBT1_HUMAN and RP11-185C18.1. GLP or GEF-like protein is another name given to CLLL7 because of its important role in the exchange of GDP/GTP. The protein has been extensively studied, and some of its functions are still under research.

Source

Rabbit

Specificity

The endogenous levels of total RCBTB1 can be detected using the RCBTB1 antibody.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Dog;Horse

Application

Western blotting (WB) is typically performed using dilutions ranging from 1:500 to 1:3000. Immunohistochemistry (IHC) involves dilutions of 1:50 to 1:200, while immunofluorescence/immunocytochemistry (IF/ICC) typically requires dilutions of 1:100 to 1:500. For ELISA assays using peptide antigens, dilutions of 1:20000 to 1:40000 are commonly used. It is important to note that these dilution ranges may vary depending on the specific antibody and experimental conditions.

Immunogen

A synthesized peptide derived from human RCBTB1.

Properties

Concentration

1mg/ml

Purification

To obtain purer antiserum, SulfoLink™ Coupling Resin was utilized in peptide affinity chromatography during the purification process.

Clonality

Polyclonal Antibody

Stability & Storage

For one year, store the product at a temperature of -20 °C. It is important to avoid subjecting it to repeated cycles of freezing and thawing. Make sure to follow these guidelines to maintain the product's quality.

Storage buffer

This solution consists of Rabbit IgG in phosphate buffered saline, with a pH of 7.4 and added 150mM of NaCl, as well as 0.02% sodium azide and 50% glycerol. It is recommended to store this solution at -20°C and it remains stable for up to 12 months from the date of receipt.

Target

Background

Cell cycle regulation may involve the remodeling of chromatin as part of its mechanism.

Tissue specificity

Expressed ubiquitously, this protein can be found in various tissues throughout the body according to PubMed articles such as PubMed:11306461 and PubMed:27486781. Specifically, in the retina, it is primarily present in the nerve fiber layer with lesser amounts being detected in the inner and outer plexiform layers, as stated in a study published on PubMed:27486781.

Celluar localization

Nucleus;

UniPort

Q8NDN9