Rabbit Anti-Retinoic Acid Receptor Beta Polyclonal Antibody #abs130185

abs130185-50ug

1-2 Weeks

201

abs130185-100ug

1-2 Weeks

301

Description

RARB is a nuclear receptor superfamily member and acts as a receptor for retinoic acid, an influential mammalian morphogen and teratogen that has significant impacts on vertebrate development. Its function involves the direct regulation of gene expression, controlling cell activity. The structure of RARB includes three domains: a modulating N-terminal domain, a DNA-binding domain, and a C-terminal steroid-binding domain.

Other names

Rearranged content:
Nuclear receptor subfamily 1 group B member 2 (NR1B2), also known as RAR beta or RAR epsilon, is a HBV-activated protein (HAP). It is encoded by the RARB gene in humans. RAR beta plays a crucial role in the cellular response to retinoic acid, a derivative of vitamin A. There are multiple isoforms of RAR beta, including RAR-beta, RAR-epsilon, RARB_HUMAN, and RRB2. These isoforms, such as Retinoic Acid Receptor Beta 2, Beta 4, and Beta 5, are polypeptides that function as transcription factors, regulating gene expression in various biological processes. The RARB gene and its protein products have been extensively studied due to their involvement in developmental processes, differentiation, and carcinogenesis.

Source

Rabbit

Specificity

The Retinoic Acid Receptor beta antibody is able to identify the total amount of Retinoic Acid Receptor beta present in the body at its natural level.

Species Reactivity

Human;Mouse

Predictive reaction species

Rabbit;Sheep;Dog;Bovine;Horse

Application

The suggested dilutions for Western blotting (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the recommended dilutions are 1:50 to 1:200. When performing immunofluorescence or immunocytochemistry (IF/ICC), it is advisable to use dilutions ranging from 1:100 to 1:500. Finally, for ELISA utilizing peptides, the suggested dilutions are between 1:20000 and 1:40000. It is important to note that these dilutions are based on the original text information and have been rearranged to provide a similar content.

Immunogen

ββ。β。，β。β，A。，β。

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized in purifying the antiserum through peptide affinity chromatography. This process ensures that the resulting antiserum is highly pure and enriched.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the quality and longevity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is advisable to avoid exposing the product to multiple freeze/thaw cycles, as this may compromise its efficacy. Taking these precautions will help maintain the integrity of the product and ensure its effectiveness when needed.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH level of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the product at a temperature of -20 °C for optimal stability, and it can remain stable for up to 12 months from the date of receipt.

Target

Background

Retinoic acid, a hormone-like molecule, binds to specific receptors in the body. These receptors, known as retinoic acid receptors (RARs), form heterodimers with retinoid X receptors (RXRs) when they are activated by their ligands: all-trans or 9-cis retinoic acid. Together, these receptor complexes play a crucial role in regulating gene expression in various biological processes.
The binding of RXR/RAR heterodimers to the retinoic acid response elements (RARE) is an important step in their gene regulatory function. RAREs consist of repeated 5'-AGGTCA-3' sequences known as DR1-DR5. When RXR/RAR complexes bind to these elements, they can activate the expression of target genes.
Interestingly, the presence or absence of hormone ligands determines the activity of RXR/RAR heterodimers. In the absence of ligands, these receptors act mainly as gene activators because their binding to corepressors is weak. However, when hormone ligands are present, the activity of RXR/RAR heterodimers can be modulated, leading to changes in gene expression.
One specific function of RXR/RAR heterodimers, when working in conjunction with another retinoic acid receptor called RARG, is the regulation of skeletal growth, matrix homeostasis, and growth plate function. This highlights the critical role of these receptor complexes in proper bone development and maintenance.
In summary, retinoic acid receptors play a crucial role in gene regulation by binding to their ligands and forming heterodimers with RXRs. Through their interaction with specific DNA sequences, RXR/RAR heterodimers are involved in various biological processes, including skeletal growth and homeostasis.

Celluar localization

Nucleus;

UniPort

P10826