Rabbit Anti-Acetyl-NF-kappaB P65 (Lys310) Polyclonal Antibody#abs130615

abs130615-50ug

1-2 Weeks

201

abs130615-100ug

1-2 Weeks

301

Description

NF-kappa-B is a versatile transcription factor that exists in virtually all cells and contributes to a range of biological functions, including inflammation, immunity, cell differentiation, growth, programmed cell death, and tumor formation. Its activity can be modulated by a variety of stimuli, making it a key player in the cellular response to internal and external stressors. Overall, NF-kappa-B is a critical regulator of many biological processes and provides a molecular mechanism for cells to rapidly and coordinately respond to changing environmental conditions.

Other names

The Avian reticuloendotheliosis viral (v rel) oncogene homolog A, also known as NFKB3 or p65, is a transcription factor that plays a critical role in the regulation of immune responses and inflammation. This protein is encoded by the NF-kappa-B p65 subunit gene, which is expressed in various cells and tissues throughout the body.
The p65 protein forms a heterodimer with other NF-kappa-B proteins and binds to DNA to regulate the expression of a broad range of genes involved in cell growth, differentiation, and survival. As a member of the NF-kappa-B family of transcription factors, p65 is activated by a wide variety of stimuli, including cytokines, pathogens, and oxidative stress.
Mutations in the NFKB3 gene are associated with a number of diseases, including inflammatory bowel disease, autoimmune disorders, and cancer. Dysregulation of NF-kappa-B signaling is also implicated in the pathogenesis of many other diseases, such as diabetes, atherosclerosis, and neurodegenerative disorders.
Research on p65 and other NF-kappa-B proteins continues to shed light on their complex roles in immune function and disease. Understanding these mechanisms may lead to new therapeutic targets and strategies for treating a wide range of health conditions.

Source

Rabbit

Specificity

The Antibody for Acetyl-NF-kappaB p65 (Lys310) specifically identifies the acetylated form of NF-kappaB p65 protein at lysine310. It is capable of detecting the endogenous levels of total NF-kappaB p65 protein only under these conditions. To summarize, this antibody is designed to recognize and measure the presence of acetylated NF-kappaB p65 protein at lysine310.

Application

For WB, the recommended dilution range is 1:500 to 1:2000, while for IHC, the dilution range is recommended to be 1:50 to 1:200. The recommended dilution for IF is 1:200, and for ELISA (peptide), the recommended dilution range is 1:20,000 to 1:40,000. It is important to note that these dilution ranges are based on the original text information and should be followed carefully for optimal results.

Immunogen

Properties

Concentration

Purification

affinity purification.

Clonality

Polyclonal Antibody

Stability & Storage

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide.

Target

Background

 NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

Celluar localization

Cytosol;Nucleus;

UniPort

Q04206