Phospho-Estrogen Receptor- Alpha (Ser167) Rabbit Polyclonal Antibody#abs130685

abs130685-50ug

1-2 Weeks

201

abs130685-100ug

1-2 Weeks

301

Description

ER-alpha is a transcription factor and nuclear hormone receptor that plays a crucial role in regulating gene expression. It has the ability to impact cellular differentiation and proliferation within targeted tissues. The isoforms of this receptor have been classified into two splice variants.

Other names

ER alpha, also known as Estrogen receptor alpha (ESR1), is a crucial protein that plays a pivotal role in regulating the function of estrogen. This protein is commonly found in the epithelia of the reproductive organs, mammary glands, and bones. It is a nuclear receptor subfamily 3 group A member 1 that binds to estrogens with high affinity and regulates gene expression. ER alpha is responsible for estrogen-mediated processes such as cell proliferation, differentiation, and maintenance of bone integrity. Its ability to signal through various pathways has also been associated with the development and progression of breast cancer. Understanding the functions and mechanisms of this protein is crucial in developing targeted therapies for estrogen-related diseases.

Source

Rabbit

Specificity

The Phospho-Estrogen Receptor- alpha (Ser167) Antibody is designed to specifically detect Estrogen Receptor- alpha at the site of Serine 167 phosphorylation. It recognizes endogenous levels of the receptor only when phosphorylated at this specific site. The antibody is a valuable tool for studying the phosphorylation status of Estrogen Receptor- alpha and its downstream signaling pathways. Its specificity ensures accurate detection of post-translational modifications, providing researchers with a deeper understanding of the receptor's complex regulatory network.

Application

The recommended dilution ratios for using this antibody in various applications are as follows: for Western blotting, dilute at 1:500-1:2000; for immunohistochemistry, dilute at 1:50-1:200; for immunofluorescence/immunocytochemistry, dilute at 1:100-1:500; for ELISA (peptide), dilute at 1:20000-1:40000. These dilution ratios will help to ensure optimal performance and accurate results when using this antibody in your experiments. It is important to note that these dilution ratios may vary depending on the specific experimental conditions and sample types being used, so it is recommended to perform some optimization experiments to determine the ideal dilution ratio for your specific application.

α167，，。ChapGPT，。

Properties

1mg/ml

Purification

This antibody was produced by purifying rabbit serum using a two-step process involving affinity chromatography on both phospho- and non-phospho-peptide affinity columns. The resulting product is highly pure and specific for its intended target. It is a valuable tool for research in various fields including immunology and cell biology.

Clonality

Polyclonal Antibody

To maintain the quality of this product, it is advisable to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles, as this could potentially compromise its effectiveness.

Storage buffer

Phosphate buffered saline solution containing Rabbit IgG is formulated with a pH of 7.4 and consists of 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C. The product is expected to maintain stability for a period of 12 months starting from the date of receipt.

Target

Background

 Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Tissue specificity

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

Celluar localization

Golgi apparatus;Nucleus;Plasma Membrane;

P03372