Phospho-Histone H3.1 (Ser10) Rabbit Polyclonal Antibody#abs130983

abs130983-50ug

1-2 Weeks

201

abs130983-100ug

1-2 Weeks

301

Description

The H3F3A variant histone H3 is known for its ability to replace the conventional H3 in a wide range of nucleosomes found in active genes. It is the predominant form of histone H3 in cells that are not actively dividing, and interestingly, it can be incorporated into chromatin without the need for DNA synthesis.

Other names

H3 histone family, member A is also known as H3/A, H31_HUMAN, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, Histone cluster 1, H3a, and many other names. It belongs to the H3 histone family and plays an important role in gene regulation and packaging the DNA molecule within the nucleus.
Another member of the H3 histone family is H3 histone family, member B, which is known as H3 histone family, member C, H3 histone family, member D, H3 histone family, member F, H3 histone family, member H, H3 histone family, member I, H3 histone family, member J, H3 histone family, member K, H3 histone family, member L, and various other names. These histone proteins are highly conserved and are involved in maintaining the structure and stability of chromatin.
There is another subfamily within the H3 histone family called H3.3A. It includes H3.3B and H3.3. These histone proteins are variants of H3 and are known for their role in transcriptional regulation, DNA repair, and epigenetic modifications.
Histone gene cluster 1 is also a part of the H3 histone family and includes members such as H3 histone family, member E, H3 histone family, member H, H3 histone family, member I, H3 histone family, member J, and many others. These histone proteins are involved in packaging DNA into chromatin, which is essential for gene regulation and chromosome stability.
In summary, the H3 histone family consists of various members such as H3/A, H3/B, H3/C, H3/D, H3/F, H3/H, H3/I, H3/J, H3/K, H3/L, H3/T, H3.3A, H3.1, H3F3A, H3FA, H3FB, H3FC, H3FD, H3FH, H3FI, H3FK, H3F1K, H3F3A, H3.3B, and more. These histone proteins play critical roles in regulating gene expression and maintaining chromatin structure.

Source

Rabbit

Specificity

Phospho-Histone H3.1 (Ser10) Antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Serine 10.

Predictive reaction species

Bovine

Application

 WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Serine 10.

Properties

1mg/ml

Purification

The antibody is  from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Tissue specificity

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

Celluar localization

Extracellular region or secreted;Nucleus;

P68431/Q71DI3/P84243