Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody#abs130987

abs130987-50ug

1-2 Weeks

201

abs130987-100ug

1-2 Weeks

301

Description

The activation and phosphorylation of Smad3, a transcription factor, occurs through TGF-beta-type receptors. Smad3 functions as a receptor-regulated protein (R-smad) and exhibits a direct binding to consensus DNA-binding elements present in the promoters of target genes. This binding event ultimately leads to the activation of these target genes. In mice, Smad3 is essential for the establishment of the mucosal immune response and plays a critical role in the proper development of the skeletal system. By rearranging the provided information, a highly similar content can be generated.

Other names

There are various names for SMAD3 such as hMAD-3, LDS1C, JV15-2 and Mothers against decapentaplegic homolog 3. It is a protein that is part of the SMAD family and is involved in cell signaling. This protein is encoded by the MADH3 gene and is known to play a crucial role in the TGF-beta signaling pathway. It interacts with other proteins like SMAD4 to form complexes that translocate to the nucleus and regulate gene expression. SMAD3 is essential for various processes like embryonic development, immune response, wound healing, and cancer progression. Dysfunction or mutations in this gene is associated with various diseases like pulmonary fibrosis, autoimmune disorders, and cardiovascular diseases. SMAD3 is a promising target for various therapeutic interventions.

Source

Rabbit

Specificity

The Phospho-Smad3 (Ser425) Antibody has been designed to specifically detect Smad3 levels that have been phosphorylated at Serine 425. This antibody is highly sensitive and can precisely detect endogenous Smad3 levels when they are phosphorylated at the mentioned site. Researchers can use this antibody to explore Smad3 phosphorylation events and gain insights into the molecular mechanisms that drive cellular responses. By detecting phosphorylation at Serine 425, this antibody can help shed light on the signaling pathways that regulate Smad3 activity and potentially identify therapeutic targets for various diseases.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

，，：
ELISA（） 1：20000-1：40000，IF/ICC 1：100-1：500，IHC 1：50-1：200，WB 1：500-1：2000。

A peptide synthesized from human Smad3 has been developed that focuses on the phosphorylation site of Serine 425. In order to create a content that is highly similar to the original text, the original information must be rearranged effectively. Thus, a synthesized peptide based on human Smad3 has been developed with a focus on the Serine 425 phosphorylation site.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to frequent cycles of freezing and thawing.

Storage buffer

The recommended storage conditions for Rabbit IgG are at -20°C in a solution of phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures the stability of the antibody for up to 12 months from the date of receipt.

Target

Background

 Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P84022