Phospho-XIAP (Ser87) Rabbit Polyclonal Antibody#abs130993

abs130993-50ug

1-2 Weeks

201

abs130993-100ug

1-2 Weeks

301

Description

The gene under consideration encodes for a particular protein that belongs to a group of proteins known for their ability to obstruct apoptosis by forming a bond with TRAF1 and TRAF2. BIRC4 is quite analogous to API1 in that it counteracts apoptosis activated by a highly reactive free radical generator called menadione, and ICE.

Other names

Baculoviral IAP repeat containing 4, also known as API3 or Apoptosis Inhibitor 3, is a protein involved in the regulation of apoptosis. It is a member of the inhibitor of apoptosis (IAP) family and is encoded by the BIRC4 gene. The protein functions as an E3 ubiquitin-protein ligase, specifically belonging to the X-linked inhibitor of apoptosis (XIAP) subfamily.
XIAP is a mammalian IAP homologue and plays a crucial role in inhibiting programmed cell death or apoptosis. It is characterized by multiple baculoviral IAP repeats (BIRs) and a RING finger domain for E3 ligase activity. XIAP acts by directly binding and inhibiting caspases, the enzymes responsible for initiating the apoptotic process. It prevents caspase activation and subsequent apoptosis by blocking the formation of the caspase activation complex.
The X-linked inhibitor of apoptosis protein (XIAP) is associated with various cellular processes, including cell survival, proliferation, and immune response regulation. Mutations in the BIRC4 gene result in X-linked lymphoproliferative syndrome type 2 (XLP2), a rare genetic disorder characterized by defects in immune system function and increased susceptibility to infections.
Understanding the function and regulation of XIAP and related proteins like API3 is important for developing therapeutic interventions targeting abnormal apoptosis in various diseases, including cancer. Further studies aimed at elucidating the precise mechanisms of XIAP action may lead to the development of novel strategies for enhancing apoptosis in cancer cells and overcoming their resistance to standard treatments.

Source

Rabbit

Specificity

The detection capability of the Phospho-XIAP (Ser87) Antibody is specific to endogenous levels of XIAP that are phosphorylated at Serine 87. To reiterate, this antibody is designed to recognize and target the phosphorylation event occurring at Serine 87 of the XIAP protein. It is important to note that the antibody will not detect non-phosphorylated XIAP or XIAP phosphorylated at other residues.

Predictive reaction species

Pig;Rabbit;Sheep;Horse;

Application

Here are some alternative ways to express the same information:
- To detect the protein of interest, a dilution range of 1:500-1:2000 is recommended for Western blotting, 1:50-1:200 for immunohistochemistry, 1:100-1:500 for immunofluorescence or immunocytochemistry, and 1:20000-1:40000 for ELISA using a peptide antigen.
- Depending on the application, different antibody dilutions can be used to target the protein of interest. For example, a suggested dilution range is 1:500-1:2000 for Western blotting, 1:50-1:200 for immunohistochemistry, 1:100-1:500 for immunofluorescence or immunocytochemistry, and 1:20000-1:40000 for ELISA using a peptide antigen.
- For optimal detection of the protein under investigation, we recommend trying different dilutions of the antibody. Typically, a dilution of 1:500-1:2000 is suitable for Western blotting, 1:50-1:200 for immunohistochemistry, 1:100-1:500 for immunofluorescence or immunocytochemistry, and 1:20000-1:40000 for ELISA with a peptide antigen.
- The protein of interest can be detected using various immunological techniques, each requiring different dilutions of the antibody. Western blotting can be performed using an antibody diluted between 1:500 and 1:2000, while immunohistochemistry requires a range of 1:50-1:200. For immunofluorescence or immunocytochemistry, a dilution of 1:100-1:500 is recommended. ELISA using a peptide antigen is typically performed with an antibody dilution of 1:20000-1:40000.

XIAP is a human protein that can be a source for synthesizing a peptide. This synthetic peptide would be specifically derived from XIAP and centered around the phosphorylation site of Serine 87. To create a similar content, rearranging the wording and structure to maintain the essence of the original text, we can say:
"Incorporating the phosphorylation site of Serine 87, a synthesized peptide can be developed from human XIAP. This artificial peptide will be derived specifically from the XIAP protein and focused on the aforementioned phosphorylation site."

Properties

1mg/ml

Purification

Our ability to create highly specific antibodies has been greatly enhanced by the use of purified rabbit serum and sequential chromatography on phospho- and non-phospho-peptide affinity columns. This technique allows us to isolate and purify the desired antibody, resulting in a product of utmost quality.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for up to one year. To prevent any damage to the product, avoid subjecting it to repeated cycles of freezing and thawing. Keep in mind that proper storage is crucial in preserving the efficacy of the product. Hence, always adhere to the recommended storage conditions to ensure its optimal performance.

Storage buffer

-20°CIgG，pH 7.4，150mM NaCl，0.02％50％。，12。，。

Target

Background

 Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.

Tissue specificity

Ubiquitous, except peripheral blood leukocytes.

Celluar localization

Cytosol;Nucleus;

P98170