Phospho-AFX (Ser197) Rabbit Polyclonal Antibody#abs130995

abs130995-50ug

1-2 Weeks

201

abs130995-100ug

1-2 Weeks

301

Description

AFX1 is a transcription factor known as FOXO4, which contains a single fork-head domain. It has been suggested that FOXO4 may play a crucial role in the insulin signaling pathway. Interestingly, this transcription factor is also implicated in acute leukemias through a chromosomal translocation referred to as t(X;11)(q13;q23), where MLLT7 and MLL/HRX genes are involved.

Other names

Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7, commonly known as AFX, AFX1, or Afxh, is a gene located on the X chromosome. It encodes for the Fork head domain transcription factor AFX1, which is also referred to as Forkhead box O4 (FOXO4) or Foxo4. Other aliases for this gene include FOXO4_HUMAN, MGC117660, MGC120490, and RGD1561201. AFX belongs to the Forkhead box family of transcription factors and plays a crucial role in various cellular processes. Additionally, AFX has been implicated in the Mixed lineage leukemia, translocated to, 7 (MLLT7) gene fusion associated with certain cancer types.

Source

Rabbit

Specificity

The Phospho-AFX (Ser197) Antibody is designed to specifically recognize and measure the levels of endogenous AFX protein only if it is phosphorylated at Serine 197. By targeting this specific phosphorylation site, the antibody allows researchers to detect and study the phosphorylation status of AFX in various biological samples. This information is crucial in understanding the regulatory mechanisms and functional implications of AFX phosphorylation at Serine 197.

Predictive reaction species

Pig;Sheep;Dog;Bovine;Horse

Application

The recommended dilution for Western blotting is 1:500-1:2000, for immunohistochemistry it is 1:50-1:200, for immunofluorescence/immunocytochemistry it is 1:100-1:500, and for ELISA using peptide it is 1:20000-1:40000.

One possible way to generate a highly similar content by rearranging the above content is:
A peptide synthesized from the human protein AFX was designed to target the phosphorylation site of Serine 197. This amino acid residue is crucial for the regulation of AFX function, as it is a target for several kinases that activate or inhibit AFX signaling pathways. By mimicking the phosphorylated state of Serine 197, the synthesized peptide can modulate AFX activity and potentially impact cellular processes such as apoptosis, differentiation, and metabolism. The development of such peptides is a promising approach for drug discovery and therapeutic intervention in diseases where AFX dysregulation has been implicated, such as cancer, diabetes, and neurodegenerative disorders.

Properties

1mg/ml

Purification

This specific antibody was obtained from rabbit serum that had been purified using a process called affinity purification. This was achieved by running the serum through a series of chromatography columns that had been constructed using both phospho- and non-phospho-peptide materials. The end result was an antibody that exhibited very high levels of similarity to the original serum, and which was ideally suited for a range of diagnostic and therapeutic applications.

Clonality

Polyclonal Antibody

One year storage at -20 °C is recommended to prevent degradation. It is important to avoid subjecting the product to freezing and thawing cycles repeatedly.

Storage buffer

The storage condition for Rabbit IgG is in phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for a period of 12 months starting from the day of receipt.

Target

Background

 Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Celluar localization

Cytosol;Nucleus;

P98177