Rabbit Anti-Histone H2B Polyclonal Antibody #abs130597

abs130597-50ug

1-2 Weeks

201

abs130597-100ug

1-2 Weeks

301

Description

The H2BFS protein is an integral part of the nucleosome, which is a fundamental unit of chromosomal DNA organization. Comprised of two molecules each of histones H2B, H2A, H3, and H4, the octameric nucleosome coils around roughly 147 base pairs of DNA. By compacting and wrapping DNA into chromatin, nucleosomes help restrict access to the genetic material by cellular machinery that requires DNA as a template. Histones are consequently central to many crucial cellular processes, including transcription regulation, DNA repair, DNA replication, and maintaining chromosomal stability.

Other names

There are numerous types of Histone H2B, including H2B K, H2B type 12, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/j, H2B/n, H2B/q, H2B/r, H2B/s, HIRA-interacting protein 1, and HIRA-interacting protein 2. Other known variations include Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-J, Histone H2B type 1-K, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 1-O, Histone H2B type 2-E, Histone H2B type 2-F, Histone H2B type 3-B, Histone H2B type F-S, Histone H2B-GL105, Histone H2B.1 A, Histone H2B.1 B, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.c, Histone H2B.d, Histone H2B.e, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.j, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.r, and Histone H2B.s.
Despite their variations, all of these types of Histone H2B play crucial roles in regulating gene expression and the stability of chromosomal DNA. They function as "spools" around which DNA winds and compacts to fit into the nucleus of a cell. Additionally, some types of Histone H2B, such as HIRA-interacting proteins, are involved in the formation and maintenance of heterochromatin, a tightly packed form of DNA that is typically inactive and regulates gene expression.
Understanding the functions of these various types of Histone H2B is critical in gaining a deeper understanding of the processes that regulate gene expression and the formation of chromosomal structure. Advancements in research and technologies that allow for more precise manipulation and analysis of these histones will continue to yield new insights and applications in both basic biological research and therapeutic development.

Source

Rabbit

Specificity

The antibody against Histone H2B can detect the levels of Histone H2B that occur naturally in the body. To create similar content, one could rephrase the statement as follows: "The presence of Histone H2B within the body can be accurately detected through the use of an antibody designed specifically for this purpose."

Species Reactivity

Human;Mouse

Application

The dilution range for Western Blot (WB) is 1:500 to 1:2000, for Immunohistochemistry (IHC) it is 1:50 to 1:200, for Immunofluorescence/Immunocytochemistry (IF/ICC) it is 1:100 to 1:500, and for ELISA (peptide) it is 1:20000 to 1:40000. Please rearrange the above information to create content that is highly similar to the original text.

Immunogen

A peptide synthesized from human Histone H2B, known as a synthesized peptide, can be generated by rearranging the original content. It is important to note that the generated content should be based on the information provided in the original text.

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimal conditions, the recommended storage temperature for one year is -20 °C. It is important to avoid subjecting the product to multiple freeze/thaw cycles to ensure its integrity.

Storage buffer

This rabbit IgG is suspended in phosphate buffered saline at a pH of 7.4 with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To ensure maximum longevity, please store this product at a temperature of -20°C. This IgG solution is guaranteed to maintain its quality for a period of 12 months from the date it is received. Ensure proper storage and handling to preserve its integrity.

Target

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Posttranslational modification

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Celluar localization

Cytosol;Extracellular region or secreted;Nucleus;

UniPort

P57053