Rabbit Anti-PFKFB2 Polyclonal Antibody #abs130279

abs130279-50ug

1-2 Weeks

201

abs130279-100ug

1-2 Weeks

301

Description

This particular enzyme, PFKFB2, serves as a bifunctional heterodimeric enzyme that plays a crucial role in both the creation and breakdown of fructose-2,6-bisphosphate. This regulatory molecule is responsible for controlling glycolysis in eukaryotes. While a closely related enzyme encoded by a separate gene is responsible for regulating fructose-2,6-bisphosphate levels in the liver and muscle, PFKFB2 regulates these levels within the heart. With its important regulatory functions, this enzyme is critical for the proper functioning of human metabolism.

Other names

Fructose 2,6 bisphosphatase, also known as 6-P2ase 3 or 6PF-2-K/Fru-2, is an enzyme involved in the regulation of glucose metabolism. Specifically, it acts as both a kinase and a phosphatase, controlling the levels of fructose 2,6-bisphosphate in the cell. This enzyme exists in different isoforms, including the brain/placenta type and the inducible type.
The brain/placenta-type isoform, also known as 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme, plays a role in brain and placental tissues. It is responsible for catalyzing the conversion of fructose 6 phosphate to fructose 2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1 (PFK-1), which is a key enzyme in glycolysis.
On the other hand, the inducible isoform, referred to as iPFK 2 or PFK/FBPase 3, is involved in response to various cellular signals. It can be induced by hormones, such as insulin and glucagon, and it acts as a regulator of glucose metabolism in various tissues, including liver, muscle, and adipose tissue.
This enzyme is of particular interest in the field of cancer research, as it has been found to be overexpressed in several types of cancer, including renal carcinoma. In fact, it is associated with a renal carcinoma antigen known as NY-REN-56, or renal carcinoma antigen NY REN 56. The exact role of fructose 2,6 bisphosphatase in cancer development and progression is still being investigated, but its dysregulation in cancer cells suggests its potential as a therapeutic target.
In summary, fructose 2,6 bisphosphatase is a multifunctional enzyme involved in glucose metabolism regulation. It exists in different isoforms, with distinct tissue-specific roles. Its dysregulation is implicated in cancer, making it an area of interest in cancer research.

Source

Rabbit

Specificity

Endogenous levels of total PFKFB2 can be detected using the PFKFB2 antibody. This means that the antibody is capable of identifying and detecting all forms of PFKFB2 that are naturally present in the body. Through this process, it becomes possible to study the activity and function of PFKFB2 in various biological systems. The accurate detection of total PFKFB2 levels is essential for understanding its role in metabolic pathways and its potential significance as a therapeutic target in disease.

Species Reactivity

Human;Mouse

Application

For Western blotting, the recommended dilution range is 1:500 to 1:3000. When used for immunofluorescence or ICC, a dilution range of 1:100 to 1:500 is suggested. For ELISA with peptide, a dilution range of 1:20000 to 1:40000 is recommended. It is important to carefully follow the recommended dilution guidelines to ensure accurate and reliable results.

Immunogen

A synthesized peptide derived from human PFKFB2.

Properties

Concentration

1mg/ml

Purification

SulfoLink™。

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is important to store it at a temperature of -20 °C for a period of one year. Avoid subjecting it to frequent freeze and thaw cycles as this may cause damage to the product.

Storage buffer

The solution contains Rabbit IgG in a phosphate buffered saline solution with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide and 50% glycerol. This solution should be stored at a temperature of -20 °C and is stable for up to 12 months from the date of receipt.

Target

Background

Fructose 2,6-bisphosphate undergoes both synthesis and degradation processes in the cell. The synthesis of fructose 2,6-bisphosphate involves specific enzymatic reactions that convert fructose 6-phosphate and ATP into fructose 2,6-bisphosphate. This process is catalyzed by the enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB). Fructose 2,6-bisphosphate acts as a key regulator of glycolysis and gluconeogenesis by influencing the activity of the enzyme phosphofructokinase-1 (PFK-1).
On the other hand, fructose 2,6-bisphosphate is also subject to degradation through the action of the enzyme fructose 2,6-bisphosphatase (F2,6BPase). This enzyme catalyzes the hydrolysis of fructose 2,6-bisphosphate into fructose 6-phosphate. The degradation of fructose 2,6-bisphosphate helps regulate its levels in the cell and influences the activity of PFK-1, thus controlling the flux of glucose through glycolysis and gluconeogenesis.
Overall, the synthesis and degradation of fructose 2,6-bisphosphate play crucial roles in cellular metabolism, particularly in regulating the balance between glycolysis and gluconeogenesis. These processes ensure an appropriate supply of energy and metabolic intermediates, allowing cells to adapt to changing metabolic demands.

Tissue specificity

Heart.

Posttranslational modification

Phosphorylation by AMPK stimulates activity.

Celluar localization

Cytosol;

UniPort

O60825