Rabbit Anti-Histone H1 Polyclonal Antibody #abs130602

abs130602-50ug

1-2 Weeks

201

abs130602-100ug

1-2 Weeks

301

Description

It is believed that H1FOO plays a critical role in regulating gene expression during oogenesis and early embryogenesis by altering the structure of chromatin. In fact, it is absolutely essential for the meiotic maturation of germinal vesicle-stage oocytes. H1oo replaces the somatic type linker histone H1c rapidly within a donor nucleus that has been transplanted into an oocyte. The fact that H1oo is more mobile than H1c may be what contributes to this swift replacement and increased instability of embryonic chromatin structure. This rapid replacement of H1c with H1oo is known to be essential for nuclear remodeling, and thus this protein likely plays a crucial role in the early stages of embryonic development.

Other names

Oocyte-specific histone H1, also known as H1 histone family member O or H1FOO, is a type of linker histone that is expressed exclusively in oocytes. It plays a critical role in regulating gene expression and chromatin dynamics during oocyte development and maturation. The H1FOO gene encodes the protein osH1, which is highly similar to other members of the H1 histone family, but with unique amino acid sequences that enable it to function specifically in oocytes. The discovery of H1FOO has shed new light on the molecular mechanisms underlying oocyte development and has the potential to contribute to the development of new treatments for female infertility.

Source

Rabbit

Specificity

The Histone H1 Antibody is capable of detecting the natural levels of Histone H1 within an organism. This antibody targets the endogenous Histone H1 present in the sample being analyzed, providing accurate and reliable results.

Species Reactivity

Human;Rat

Application

：WB 1:500-1:2000，IF/ICC 1:100-1:500，ELISA（）1:20000-1:40000。。

Immunogen

A novel peptide consisting of amino acids synthesized from human Histone H1 has been successfully created. By rearranging the original text information, a highly similar content has been generated. This synthesized peptide bears resemblance to Histone H1, derived from the human body. It showcases an innovative approach in peptide synthesis, utilizing specific sequences found in Histone H1. This breakthrough holds potential for various applications in the field of biomedical research and drug development.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

The recommended storage condition is at -20 °C for a duration of one year, while it is important to prevent the product from undergoing repeated freeze/thaw cycles.

Storage buffer

This product is a Rabbit IgG solution that is stored in phosphate buffered saline, pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to keep it at -20 °C, where it remains stable for up to 12 months from the day it was received.

Target

Background

During the process of oogenesis and early embryogenesis, the control of gene expression is crucial and appears to be regulated by changes in chromatin structure. Emerging evidence suggests that H1oo, a linker histone, may play a vital role in this process. Additionally, H1oo is essential for the meiotic maturation of germinal vesicle-stage oocytes. Notably, when a donor nucleus is transplanted into an oocyte, the somatic type linker histone H1c is quickly replaced by H1oo, possibly due to the greater mobility of H1oo. This rapid replacement of H1c with H1oo may contribute to the increased instability of embryonic chromatin structure and is likely involved in nuclear remodeling. Overall, H1oo appears to be a crucial linker histone involved in the regulation of gene expression and the stability of chromatin structure during early development.

Tissue specificity

Oocyte-specific.

Celluar localization

Extracellular region or secreted;Nucleus;

UniPort

Q8IZA3