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Rabbit Anti-Cyclin D1 Polyclonal Antibody #abs130610

Description

Catalog-specification	Delivery time	USD price
abs130610-50ug	1-2 Weeks	201
abs130610-100ug	1-2 Weeks	301

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Overview
Description	Members of the highly conserved cyclin family, such as the protein encoded by this gene, display a remarkable periodicity in protein abundance throughout the various stages of the cell cycle. These cyclins play a crucial role as regulators of CDK kinases, essential enzymes involved in cell cycle progression. Each cyclin exhibits its own unique pattern of expression and degradation, contributing to the precise coordination of mitotic events in a timely manner.
Other names	The B cell CLL/lymphoma 1, also known as B cell leukemia 1 or B cell lymphoma 1 protein, is a key player in regulating cell cycle progression and proliferation in normal and cancerous cells alike. The protein, which is encoded by the BCL1 gene, is commonly referred to as the BCL-1 oncogene due to its potent ability to promote cell survival and inhibit apoptosis. The CCND1 gene, which codes for the G1/S-specific cyclin-D1 protein, is frequently involved in chromosomal translocations in cancer cells, leading to the formation of fusion genes such as CCND1/FSTL3, CCND1/IGHG1, CCND1/IGLC1, and CCND1/PTH. These fusion genes result in aberrant expression of cyclin-D1, conferring a growth advantage to the affected cells and contributing to tumorigenesis. PRAD1, or parathyroid adenomatosis 1, is another name for the BCL1 gene, reflecting its original discovery in parathyroid adenomas. The U21B31 locus on chromosome 11q13, where the BCL1 gene is located, is also known as D11S287E. Other aliases for the BCL1 gene include Cyl 1 and cD1. Overall, the BCL-1 oncogene and its downstream effector cyclin-D1 represent important targets for cancer therapy, as their dysregulation contributes to the development and progression of many types of cancer. Understanding the molecular mechanisms underlying their activity is critical for the development of effective targeted therapies to treat cancer.
Source	Rabbit
Specificity	The endogenous levels of total Cyclin D1 can be detected using the Cyclin D1 Antibody. This antibody is designed to specifically target and identify Cyclin D1 protein.
Species Reactivity	Human;Mouse;Rat
Antigen	Cyclin D1
Application	These are the recommended dilutions for using this antibody: 1:500-1:2000 for Western blot, 1:50-1:200 for Immunohistochemistry, 1:100-1:500 for Immunofluorescence and Immunocytochemistry, and 1:20000-1:40000 for ELISA with peptide substrates. Please note that these dilutions are only recommendations and may need to be optimized for your specific experiment and system. It is important to follow proper experimental protocols and controls to ensure the accuracy and reproducibility of your results.
Immunogen	Cyclin D1, a key protein involved in cell cycle regulation, can serve as a source for the synthesis of a peptide with high similarity to the human version. By rearranging the above information, I aim to generate content that is still derived from the original text.
Properties
MW	33kDa
Concentration	1mg/ml
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid any instances of repeated freezing and thawing cycles, as this may compromise the integrity of the product. For best results, adhere strictly to these storage guidelines.
Purification	SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography for the purification of the antiserum. Let me generate a highly similar content by rearranging the provided information while ensuring its relevance to the original text.
Storage buffer	The storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with a pH of 7.4. It should be supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This product remains stable for a period of 12 months from the date of receipt.
Target
Background	Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Celluar localization	Cytosol;Nucleus;
UniPort	P24385