Rabbit Anti-HIF1A Polyclonal Antibodyy #abs130612

abs130612-50ug

1-2 Weeks

201

abs130612-100ug

1-2 Weeks

301

Description

Oxygen deprivation, or hypoxia, adversely affects the growth and viability of cells. In response to this condition, hypoxia-inducible factors, such as HIF-1α, Arnt 1 (also known as HIF-1β), EPAS-1 (also known as HIF-2α), and HIF-3α, are activated. These factors are part of the Per-Arnt-Sim (PAS) domain transcription factor family. Their primary role is to restore oxygen homeostasis by inducing glycolysis, erythropoiesis, and angiogenesis. By rearranging the given information, the following highly similar content is generated, while still providing accurate information from the original text.

Other names

ARNT-interacting protein, also known as HIF1-alpha or hypoxia-inducible factor 1 alpha, is a member of the PAS superfamily and a basic helix-loop-helix transcription factor. It is involved in the response to low oxygen levels, or hypoxia, and plays a critical role in regulating cellular metabolism, angiogenesis and erythropoiesis.
HIF1-alpha is also known as a member of PAS protein 1, MOP1 and PASD8. Its structure is composed of two subunits, the alpha and beta subunits. While the beta subunit is stable under all conditions, the alpha subunit is constantly degraded under normoxic conditions, or conditions with sufficient oxygen.
Under hypoxic conditions, HIF1-alpha is stabilized and forms a heterodimer with the beta subunit to activate target genes involved in hypoxia response. It is also involved in the regulation of key cellular processes such as glucose metabolism, apoptosis and cell differentiation.
Overall, ARNT-interacting protein or HIF1-alpha is an important player in the response to hypoxia, and understanding its regulatory mechanisms may provide potential targets for therapeutic interventions in hypoxia-related diseases such as cancer and ischemic diseases.

Source

Rabbit

Specificity

HIF1a antibody detects endogenous levels of HIF1a.

Species Reactivity

Pig;Rabbit;Bovine;Horse;

Application

The recommended dilutions for using this antibody in various applications are WB 1:500-1:2000, IHC 1:50-1:200, IF 1:200, and ELISA(peptide) 1:20000-1:40000. It is important to note that these dilutions may need to be altered based on factors such as sample type and experimental conditions. We suggest starting with the recommended dilutions and then adjusting as needed. It is also important to ensure that proper controls are included in each experiment to ensure accurate and reliable results. Overall, this antibody is a valuable tool for researchers in a variety of fields and applications.

Immunogen

A synthesized peptide derived from human HIF1a.

Properties

Concentration

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. An effective method for isolating the desired molecules, this process yielded a highly purified product.

Clonality

Polyclonal Antibody

Stability & Storage

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 15 months from date of receipt.

Target

Background

 Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Tissue specificity

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Celluar localization

Cytosol;Nucleus;

UniPort

Q16665