Rabbit Anti-Pim-1 Polyclonal Antibody #abs130591

abs130591-50ug

1-2 Weeks

201

abs130591-100ug

1-2 Weeks

301

Description

The proto-oncogene serine/threonine kinase Pim1 plays a key role in promoting cell survival and multiplication, thereby conferring a significant advantage in the development of tumors. Pim1 exerts its oncogenic effects through several mechanisms, including the regulation of MYC transcriptional activity, cell cycle progression, and the inhibition of proapoptotic proteins such as BAD, MAP3K5, and FOXO3. By phosphorylating MYC, Pim1 increases MYC protein stability and transcriptional activity. The strong synergism between Pim1 and MYC in promoting tumorigenesis may be partly explained by Pim1's ability to stabilize and enhance MYC function.

Other names

PIM1, also known as Oncogene PIM 1 or pim 1 kinase, is a protein that plays a role in cancer development. It belongs to the PIM family of oncogenes, which also includes Pim2 and Pim3. PIM1 is a serine/threonine protein kinase, specifically the 44 kDa isoform of the Pim 1 kinase. It is associated with the proviral integration site 1 and proviral integration site 2. PIM1_HUMAN is the gene symbol for PIM1 in humans. This oncogene has been linked to various cellular processes involved in cancer progression. Its functions are regulated by phosphorylation events and it has been identified as a potential target for cancer therapy.

Source

Rabbit

Specificity

Pim-1 Antibody detects endogenous levels of Pim-1.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilutions for WB are 1:500-1:2000, for IF/ICC are 1:100-1:500, and for ELISA using peptides are 1:20000-1:40000. To ensure optimal results, it is important to follow these dilution guidelines when using these assays. Dilutions outside of the recommended range may result in inaccurate or non-specific results, which can greatly affect the validity of the experiment. Therefore, it is important to carefully consider the appropriate dilution for each assay before proceeding with the experiment.

Immunogen

A synthesized peptide derived from human Pim-1.

Properties

Concentration

1mg/ml

Purification

We used SulfoLink™ Coupling Resin in peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the material's integrity, it is imperative to store it at -20 °C for a period of one year. Avoid exposing it to repeated freeze/thaw cycles, which may compromise its quality.

Storage buffer

The Rabbit IgG comes in a solution of phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to be stored at a temperature of -20 °C for maximum stability. This product is guaranteed to be stable for a period of 12 months from the date of receipt.

Target

Background

PIM1 is a proto-oncogene with serine/threonine kinase activity. It plays a crucial role in promoting cell survival and proliferation, making it a vital player in tumorigenesis. PIM1 exerts its oncogenic activity through several mechanisms, including regulating MYC transcriptional activity, cell cycle progression, and inhibiting proapoptotic proteins such as BAD, MAP3K5, and FOXO3.
PIM1 phosphorylates MYC, increasing protein stability and transcriptional activity. Its stabilization of MYC potentially explains the strong synergism between these two oncogenes in tumorigenesis. PIM1 also promotes survival signaling by phosphorylating BAD, which induces the release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. PIM1 also inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK, subsequently reducing caspase-3 activation and cell apoptosis.
In addition to survival signaling, PIM1 stimulates cell cycle progression at G1-S and G2-M transitions by phosphorylating CDC25A and CDC25C. It relocates CDKN1A, a regulator of cell cycle progression at G1, to the cytoplasm, enhancing its protein stability. PIM1 down-regulates expression of CDKN1B, a cell cycle regulator, at both transcriptional and post-translational levels. PIM1 phosphorylates CDKN1B, inducing 14-3-3-proteins binding, nuclear export, and proteasome-dependent degradation.
Moreover, PIM1 affects the structure and silencing of chromatin by phosphorylating HP1 gamma/CBX3. It acts as a regulator of homing and migration of bone marrow cells by functionally interacting with the CXCL12-CXCR4 signaling axis. PIM1, with its multifaceted role in cancer biology, proves to be an essential target in designing new cancer therapeutics.

Tissue specificity

Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

Posttranslational modification

Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.Ubiquitinated, leading to proteasomal degradation.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

UniPort

P11309