Rabbit Anti-Claudin2 Polyclonal Antibody #abs130065

abs130065-50ug

1-2 Weeks

201

abs130065-100ug

1-2 Weeks

301

Description

The tight junction-specific obliteration of the intercellular space is greatly influenced by claudin 2, which functions through its calcium-independent cell-adhesion activity. This protein belongs to the claudin family and has the capability to form both homo- and heteropolymers with other CLDN proteins. Interestingly, its homopolymers interact with CLDN3, but not with CLDN1. Furthermore, claudin 2 directly interacts with important proteins involved in tight junction formation and maintenance, namely TJP1/ZO-1, TJP2/ZO-2, and TJP3/ZO-3. These interactions contribute to the overall integrity and functionality of tight junctions within cells. To recapitulate, claudin 2 plays a vital role in the regulation of tight junctions, functioning independently of calcium and exhibiting specific interactions with other claudin proteins and tight junction-associated proteins.

Other names

Claudin-2 is also known as Claudin2 and is encoded by the CLD2_HUMAN gene. It is a protein that is associated with tight junctions. The full name for Claudin2 is CLDN 2. It is also referred to by other names such as CLDN2, OTTHUMP00000023793, SP 82, and SP82.

Source

Rabbit

Specificity

The total Claudin 2 levels can be effectively detected using the Claudin 2 antibody, which specifically targets the endogenous Claudin 2 protein.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

Western Blot (WB) is typically performed at dilutions ranging from 1:500 to 1:3000. For Immunohistochemistry (IHC), the recommended dilution range is 1:50 to 1:200. Immunofluorescence (IF) experiments are generally conducted at a dilution of 1:200. For ELISA using peptide antigens, it is commonly advised to dilute the sample in the range of 1:20000 to 1:40000. Please note that these dilution recommendations are based on the original information provided and should be followed for accurate results.

Immunogen

A peptide synthesized from human Claudin 2 is available. It is a high-quality product that closely resembles the original protein. The peptide can be used for various applications, including research and therapeutic purposes. Its production involves solid-phase peptide synthesis, which ensures its purity and stability. The synthesized Claudin 2 peptide is an invaluable tool for investigating the function of Claudin 2 in normal and diseased conditions. Overall, this peptide provides a reliable and efficient way to study the biology of Claudin 2.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to ensure purification.

Clonality

Polyclonal Antibody

Stability & Storage

It is recommended to keep the product at a temperature of -20°C for a period of one year. Be sure to avoid repeated cycles of freezing and thawing to maintain the quality of the product.

Storage buffer

This product is Rabbit IgG that comes in a solution containing phosphate buffered saline of pH 7.4, 150 mM NaCl, sodium azide, and glycerol. It is recommended to store this solution at a temperature of -20 °C. The shelf life of this product is 12 months from the date of receipt. To maintain product quality, it is important to store and handle the solution properly.

Target

Background

The calcium-independent cell-adhesion activity plays a crucial part in the complete closure of the intercellular space specific to tight junctions.

Posttranslational modification

Though the disulfide bond plays a crucial role in pore formation, its absence does not hinder the proper trafficking of proteins. Let me reiterate this by presenting the information in a slightly different manner.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

UniPort

P57739