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Rabbit Anti-ALOX5AP Polyclonal Antibody#abs137844

Please note that the provided price is for reference only. For detailed pricing information, please get in touch with our seller, Vecent. Feel free to reach out to Vecent for further clarification. Western blot analysis ALOX5AP using HepG2 whole cell lysates This product is for research use...

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Description

Catalog-specification	Delivery time	USD price
abs137844-100ug	1-2 Weeks	301.0
abs137844-50ug	1-2 Weeks	201.0

Please note that the provided price is for reference only. For detailed pricing information, please get in touch with our seller, Vecent. Feel free to reach out to Vecent for further clarification.

Overview
catalog	abs137844
Other names	ALOX5AP, also known as 5-lipoxygenase activating protein or FLAP, is a crucial regulator in the arachidonate 5-lipoxygenase pathway. It interacts with MK-886 and functions as a binding protein. Its primary role is to activate the enzyme 5-lipoxygenase, which initiates the conversion of arachidonic acid into leukotrienes. Leukotrienes are potent inflammatory mediators involved in various physiological and pathological processes. ALOX5AP plays a significant role in promoting leukotriene synthesis and subsequent inflammation. Additionally, it has been implicated in the pathogenesis of several inflammatory and allergic diseases. A thorough understanding of ALOX5AP and its relationship with 5-lipoxygenase provides crucial insights into the development of novel therapeutic strategies targeting the arachidonate 5-lipoxygenase pathway.
Source	Rabbit
Specificity	The endogenous levels of total ALOX5AP can be detected using the ALOX5AP Antibody. This antibody is designed to specifically recognize and bind to the total ALOX5AP protein present in the sample. By detecting the endogenous levels, researchers can gain valuable insights into the expression and regulation of ALOX5AP in various biological systems.
Species Reactivity	Human
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Bovine;Sheep;
Antigen	ALOX5AP
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human ALOX5AP.
MW	18 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography. To achieve this, the resin was used for coupling with specific peptides, thereby assisting in the isolation of the desired protein. The resulting antiserum was thus obtained in a highly purified form, with minimal impurities.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain quality, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.
Storage buffer	The Rabbit IgG is kept in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It's recommended to store at -20 °C and is stable for 12 months from the date of receipt. To ensure the preservation of the protein's integrity, it's crucial to strictly abide by the storage conditions to maintain the functionality and potency of the Rabbit IgG.
Target
Background	Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
Celluar localization	Endoplasmic reticulum;Nucleus;
UniPort	P20292