Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-PRKAA1 Polyclonal Antibody#abs135881

Description

Catalog-specification	Delivery time	USD price
abs135881-100ug	1-2 Weeks	301.0
abs135881-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For the detailed price information, kindly get in touch with our seller, Vecent. It is important to understand that the price provided is subject to change based on various factors. Our seller will be able to provide you with the most up-to-date and accurate pricing details. So, feel free to contact Vecent for any queries related to pricing or product information.

Overview
catalog	abs135881
Other names	The protein known as 5'-AMP-activated protein kinase catalytic subunit alpha-1, also referred to as AMPK alpha 1 or AAPK1, plays a crucial role in cellular energy regulation. This catalytic subunit is a key component of the AMP-activated protein kinase complex. It is involved in phosphorylating and activating enzymes that control metabolic pathways, such as acetyl CoA carboxylase and HMG CoA reductase. By doing so, it helps maintain energy homeostasis in various tissues and organs. The AMPK alpha 1 chain, encoded by the PRKAA1 gene, is an essential regulatory enzyme involved in cellular signaling and metabolism. It acts as a sensor for cellular energy status by responding to changes in AMP and ATP levels. When the energy levels are low, AMP binds to the regulatory subunits of AMPK, leading to phosphorylation and activation of the catalytic subunit. The AMPK alpha 1 chain is also implicated in the regulation of lipid metabolism. It can inhibit lipogenesis by phosphorylating and inhibiting acetyl CoA carboxylase, an enzyme involved in fatty acid synthesis. Additionally, it promotes lipolysis by phosphorylating and activating hormone-sensitive lipase, which hydrolyzes stored triglycerides into free fatty acids. Moreover, AMPK alpha 1 is associated with the modulation of protein synthesis and autophagy. It can phosphorylate and inhibit the mammalian target of rapamycin complex 1 (mTORC1), a key regulator of protein synthesis and cell growth. Furthermore, AMPK alpha 1 activation stimulates autophagy, a cellular process involved in the degradation and recycling of damaged proteins and organelles. In summary, the AMP-activated protein kinase catalytic subunit alpha-1, also known as AMPK alpha 1 or AAPK1, is a crucial protein involved in energy regulation and metabolism. Its activation is triggered by increases in AMP-to-ATP ratio, leading to the phosphorylation and activation of various enzymes that control pathways related to energy production, lipid metabolism, protein synthesis, and autophagy. Overall, AMPK alpha 1 plays a vital role in maintaining cellular energy balance and adaptive responses to metabolic stress.
Source	Rabbit
Specificity	The detection of total AMPK alpha levels in its endogenous form is accomplished using the AMPK alpha Antibody. This antibody is designed to specifically recognize and bind to the complete AMPK alpha protein, allowing for accurate determination of its presence within a biological sample. By detecting the endogenous levels of AMPK alpha, researchers can gain insights into its physiological functions and regulatory mechanisms. This information can be crucial for studying various cellular processes and developing therapeutic interventions targeted at modulating AMPK alpha activity.
Species Reactivity	Human;Mouse;Rat
Antigen	PRKAA1
Application	For Western Blotting, dilute the antibody at a ratio of 1:500 to 1:2000. For Immunohistochemistry, a dilution ratio of 1:50 to 1:200 is recommended. If you are using the antibody for Immunofluorescence, a dilution ratio of 1:100 is appropriate. For ELISA using peptide as the antigen, dilute the antibody at a ratio of 1:20000 to 1:40000. It is essential to follow these suggested dilution ratios to obtain optimal results.
Immunogen	AMPK alpha is the source from which a synthesized peptide, with high similarity, is derived in humans. Let's explore a different approach to generate unique content rather than using the ChapGPT-generated dialogue method.
MW	63kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography, resulting in a highly purified product.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Posttranslational modification	Ubiquitinated.Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	Q13131