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Rabbit Anti-AMFR Polyclonal Antibody#abs137195

Please note that the price mentioned above is for your reference only. If you require more detailed pricing information, please reach out to our sales representative Vecent. Western blot analysis AMFR using COS7 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs137195-100ug	1-2 Weeks	301.0
abs137195-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. If you require more detailed pricing information, please reach out to our sales representative Vecent.

Overview
catalog	abs137195
Other names	The AMF receptor, also known as autocrine motility factor receptor, has two isoforms: isoform 1 and isoform 2. The receptor is also referred to as AMFR or gp78. AMFR is a RING finger protein 45, also denoted as RNF 45. It functions as an E3 ubiquitin-protein ligase and plays a crucial role in protein degradation pathways. AMFR isoform 1 and isoform 2 are encoded by the same gene, and the precursor proteins of both isoforms are identical except for a few extra amino acids present in isoform 2.
Source	Rabbit
Specificity	The total AMFR level can be accurately detected by using the AMFR Antibody, which specifically targets the endogenous AMFR protein.
Species Reactivity	Human;Rat
Antigen	AMFR
Application	IHC at a dilution of 1:200, Western Blotting at dilutions ranging from 1:1000 to 1:3000, and ELISA using a peptide at dilutions of 1:20000 to 1:40000 are the recommended methods for protein analysis.
Immunogen	A synthesized peptide derived from human AMFR.
MW	73 kDa.
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography, employing SulfoLink™ Coupling Resin, was utilized to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is advised to keep it stored at a temperature of -20 °C for a period of one year. It is also recommended to avoid exposing the product to repeated freeze/thaw cycles, which can cause damage to its composition. Please ensure that you take necessary precautions while storing the product to maintain its effectiveness and longevity.
Storage buffer	The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl and 0.02% sodium azide as preservatives. Additionally, the solution is mixed with 50% glycerol for enhanced stability. It is recommended to store the product at a temperature of -20 °C. The Rabbit IgG remains stable for a period of 12 months from the date of receipt.
Target
Background	E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Celluar localization	Endoplasmic reticulum;Golgi apparatus;Nucleus;
UniPort	Q9UKV5