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Rabbit Anti-OTUB1 Polyclonal Antibody#abs139518

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Description

Catalog-specification	Delivery time	USD price
abs139518-100ug	1-2 Weeks	301.0
abs139518-50ug	1-2 Weeks	201.0

Overview
catalog	abs139518
Other names	OTUB1, also known as hOTU1 or HSPC263, is a deubiquitinating enzyme. It belongs to the OTU domain-containing family and is commonly referred to as OTB1. OTUB1 is a protein that binds to ubiquitin aldehyde and plays a role in regulating the ubiquitin system. It is involved in the removal of ubiquitin from target proteins and is thus classified as a ubiquitin-specific-processing protease. OTUB1 is also known as otubain-1 or ubiquitin thioesterase OTUB1. It is an important enzyme in the cell's protein degradation pathway. The OTU domain present in OTUB1 binds to ubiquitin, allowing OTUB1 to interact with ubiquitin-conjugated proteins. This interaction is crucial for the regulation of various cellular processes. By rearranging the above information, the following text is generated.
Source	Rabbit
Specificity	The OTUB1 Antibody is designed to recognize the total amount of OTUB1 present in the body. It is capable of detecting endogenous levels of the protein and can be used for a variety of purposes. To ensure that the generated content is based on the original text information, it is important to rearrange the words and phrases in a way that maintains the meaning of the original text.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;Horse
Antigen	OTUB1
Application	For Western blotting, dilute the primary antibody in a range of 1:1000-3000. When performing immunohistochemistry, a dilution of 1:200 for the primary antibody is recommended. For immunofluorescence and immunocytochemistry applications, dilute the antibody in a range of 1:100-1:500. To use the primary antibody in an ELISA assay with a peptide substrate, it is recommended to use a dilution range of 1:20000-1:40000. It is crucial to follow the recommended dilution ranges to achieve optimal results in your experiments.
Immunogen	A synthesized peptide derived from human OTUB1.
MW	31 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed for the purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, keep the store temperature at -20 °C for a duration of one year. It is important to avoid repeating the freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
Tissue specificity	Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	Q96FW1