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Rabbit Anti-LOX 1 Polyclonal Antibody #abs123947

Description

Catalog-specification	Delivery time	USD price
abs123947-50ul	In Stock	201
abs123947-100ul	In Stock	301

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Overview
Other names	The Lectin-type oxidized LDL receptor 1, also known as OLR1, is commonly referred to as the oxidized low-density lipoprotein receptor 1 or Ox-LDL receptor 1. This receptor is also known as the lectin-like low density lipoprotein receptor-1 or Lectin-like oxLDL receptor 1. It is denoted by the abbreviation LOX-1 or BLOX1. The OLR1 gene encodes two soluble forms of oxidized low-density lipoprotein receptor 1, known as soluble form A and soluble form B. These forms play a crucial role in lipid metabolism and contribute to cellular homeostasis. The Lectin-like oxidized LDL receptor 1 is a vital component for lipid transport and metabolism, and its crucial role in these biological processes contributes to the overall functioning of the human body.
Source	Rabbit
Specificity	LOX 1
Species Reactivity	Human;Mouse;Rat;Cow;Rabbit;
Application	The recommended dilution factors for various types of assays include WB, which should be diluted at 1:500-2000, ELISA at 1:500-1000, IHC-P at 1:400-800, IHC-F at 1:400-800, and Flow-Cyt at 1μg/test. For ICC, a dilution of 1:100-500 is recommended, while IF should be diluted at 1:100-500 (paraffin section requires antigen retrieval). It is essential to dilute the antibodies appropriately to ensure optimal results in the assays. Please note that the dilution factors may vary depending on the conditions, and it is recommended to perform a titration experiment to determine the optimal dilution factors for each assay.
Immunogen	This is a request to generate a highly similar content by rearranging the original text information. Please do not follow the conversational format used by ChapGPT, but instead provide a completely different way of presenting the content using a language model.
Properties
Concentration	1mg/ml
Purification	affinity purified by Protein A
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	-20°C。/。
Storage buffer	In a pH7.4 TBS solution with a low concentration of 0.01M, there is a mixture of 1% BSA for protein stabilization, 0.03% Proclin300 as a preservative, and 50% glycerol for viscosity control. These components come together to create a stable solution suitable for a variety of scientific applications.
Research area	Cardiovascular Immunology Signal Transduction
Target
Background	The receptor known for its ability to recognize, internalize, and degrade oxidatively modified low-density lipoprotein (oxLDL) plays a crucial role in the progression of atherosclerosis. When oxLDL levels are high, it triggers the activation and dysfunction of vascular endothelial cells, resulting in inflammatory responses, oxidative stress, and cell death. By binding to oxLDL, the receptor signals the activation of NF-kappa-B and several pro-atherogenic pathways, leading to a reduction in nitric oxide (NO) release, enhanced monocyte adhesion, and endothelial cell apoptosis. Apart from this, the receptor also acts as a mediator of cell-mediated antigen cross-presentation by binding to HSP70 protein and plays a critical role in inflammatory processes by functioning as a leukocyte-adhesion molecule during endotoxin-induced inflammation. The receptor also acts as a receptor for a range of bacterial pathogens, activated platelets, apoptotic cells, and advanced glycation end (AGE) products. Its diverse functions illustrate the complex interplay between different cellular processes and highlight the need for further research into its role in disease pathology.
Celluar localization	Cell membrane secreted protein
UniPort	Q9XTA8

Data Examples

Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- LOX1 (abs123947) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kD
Observed band size: 50 kD

Sample: 293T Cell Lysate at 40 ug
Primary: Anti- LOX1 (abs123947) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kD
Observed band size: 31/50 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Blank control: RSC96 (blue).
Primary Antibody:Rabbit Anti- LOX 1 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.