Rabbit Anti-ATM Polyclonal Antibody#abs131163

abs131163-50ug

1-2 Weeks

201

abs131163-100ug

1-2 Weeks

301

Description

The ATM gene encodes for a protein belonging to the PI3/PI4-kinase family. This particular protein plays a crucial role as a checkpoint kinase in the cell cycle, primarily by phosphorylating various downstream proteins. As a result, it acts as a key regulator for a diverse range of proteins, including tumor suppressors like p53 and BRCA1, as well as checkpoint proteins such as CHK2, RAD17, RAD9, and the DNA repair protein NBS1. Its significant involvement in the phosphorylation and regulation of these important proteins highlights its importance in maintaining proper cellular function.

Other names

ATM, also known as A-T mutated or Ataxia telangiectasia mutated, is a serine/threonine protein kinase. It plays a crucial role in maintaining genome stability by regulating cell cycle checkpoint activation, DNA repair, and apoptosis. The ATM gene is responsible for encoding this protein, and mutations in the gene can lead to Ataxia telangiectasia, a rare genetic disorder characterized by neurodegeneration and an increased risk of cancer.
The Ataxia telangiectasia mutated homolog is the counterpart of ATM in humans, while AT1, ATA, ATC, ATD, ATDC, ATE, and TELO1 are various aliases associated with this gene. Other names like DKFZp781A0353, MGC74674, OTTHUMP00000232981, Tefu, and Telomere fusion protein are also used to refer to ATM or its related proteins, but their specific functions may vary.
Overall, ATM is a crucial protein involved in maintaining genomic stability, and understanding its role and function can provide insights into cellular processes and potentially help develop targeted therapies for diseases associated with ATM mutations.

Source

Rabbit

Specificity

The total ATM protein's endogenous level is detected by the antibody.

Application

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，。（Western Blotting），1:5001:1000；（Immunohistochemistry），1:501:200；，（ELISA），1:200001:40000。
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(EGSQS)，：SGQSE
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Properties

1mg/ml

Purification

In order to purify the antiserum, SulfoLink™ Coupling Resin was utilized in peptide affinity chromatography. By rearranging the given information, a highly similar content can be generated as follows: The purification process of the antiserum involved utilizing SulfoLink™ Coupling Resin in peptide affinity chromatography.

Clonality

Polyclonal Antibody

It is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles.

Storage buffer

The content is supplied in a solution of phosphate buffered saline, pH 7.4, containing 150mM NaCl. It does not contain Mg2+ and Ca2+. Additionally, it contains 0.02% sodium azide and 50% glycerol.

Target

Background

This protein kinase is responsible for activating checkpoint signaling in response to various types of DNA damage, such as double strand breaks, apoptosis, and genotoxic stresses like UVA light. It detects these damages by recognizing specific substrate sequences. One important function of this kinase is to phosphorylate the histone variant H2AX/H2AFX at double strand breaks and regulate DNA damage response mechanisms. It also plays a role in pre-B cell allelic exclusion, preventing recombination of the second allele. Additionally, it is involved in signal transduction and cell cycle control, and may act as a tumor suppressor. This kinase phosphorylates a number of other proteins, including DYRK2, FANCD2, NFKBIA, and p53/TP53, and may be involved in vesicle and protein transport, T-cell development, and neurological functions. Another function is in replication-dependent histone mRNA degradation. It binds to DNA ends and phosphorylates ATF2 to stimulate its function in DNA damage response. When responding to genotoxic stress, it prevents the MDM2-mediated ubiquitination and subsequent proteasome degradation of DYRK2 in the nucleus. Overall, this protein kinase is crucial for maintaining DNA integrity and proper cellular function.

Tissue specificity

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Celluar localization

Cytoskeleton;Nucleus;

Q13315