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Rabbit Anti-HP1gamma Polyclonal Antibody#abs140445

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Description

Catalog-specification	Delivery time	USD price
abs140445-100ug	1-2 Weeks	301.0
abs140445-50ug	1-2 Weeks	201.0

Overview
catalog	abs140445
Other names	Gamma chromobox protein homolog 3, also known as CBX3 or HP1 gamma homolog, is a crucial component involved in the regulation of gene expression and chromatin structure. This protein, encoded by the CBX3 gene, plays a role in maintaining heterochromatin integrity and has been linked to various biological processes. CBX3, also referred to as CBX3_HUMAN, is a member of the chromobox protein family, characterized by the presence of a chromodomain. This chromodomain is responsible for binding to methylated lysine residues on histones, facilitating the formation of heterochromatin. As a result, CBX3 is involved in the organization and compaction of DNA within the nucleus. Another name associated with CBX3 is HECH, which stands for heterochromatin-like protein 1. This name reflects the protein's ability to mimic the characteristics of heterochromatin and influence chromatin structure and function. CBX3 is also recognized as a modifier 2 protein, indicating its role in modifying cellular processes through interactions with other proteins or DNA elements. Through these interactions, CBX3 can influence gene expression patterns and contribute to the regulation of various biological pathways. In summary, CBX3, prominently known as HP1 gamma, is a versatile protein involved in maintaining heterochromatin structure, modulating gene expression, and participating in diverse cellular processes.
Source	Rabbit
Specificity	The detection of endogenous levels of total HP1gamma is possible using the HP1gamma Antibody. By utilizing this antibody, researchers can accurately measure the amount of HP1gamma present within a biological sample. This valuable tool provides insights into the expression levels and localization patterns of HP1gamma, contributing to a deeper understanding of its role in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Horse;Bovine
Antigen	HP1gamma
Application	The recommended working dilution for Western blotting is 1:1000. For immunofluorescence or immunocytochemistry, the suggested dilution range is 1:100 to 1:500. When using ELISA with peptides, the optimal dilution range is 1:20000 to 1:40000. These dilution guidelines are based on the original text information and are crucial for obtaining accurate and reliable results from experiments. It is essential to carefully follow the recommended dilution ranges to ensure the success of your experiments.
Immunogen	A synthesized peptide derived from human HP1gamma.
MW	22kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles. Taking these precautions will help preserve the product's integrity and effectiveness.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Posttranslational modification	Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	Q13185