Rabbit Anti-Claudin1 Polyclonal Antibody #abs130064

abs130064-50ug

In Stock

201

abs130064-100ug

In Stock

301

Description

Claudin 1 is a vital component of tight junctions, which play a crucial role in maintaining the integrity of the intercellular space. It achieves this through its calcium-independent cell-adhesion activity. As a co-receptor for HCV entry into hepatic cells, it has proven to be an important protein in liver pathology. This protein belongs to the claudin family and can form homo- and heteropolymers with other CLDN. Interestingly, homopolymers can interact with CLDN3, but not CLDN2 homopolymers. Claudin 1 directly interacts with TJP1/ZO-1, TJP2/ZO-2, and TJP3/ZO-3, as well as MPDZ and INADL. Additionally, it may also interact with HCV E1 and E2 proteins, further establishing its significance in the viral pathogenesis.

Other names

1. Senescence-associated epithelial membrane protein 1, also known as Claudin-1 or CLDN1, is a protein encoded by the CLDN1 gene in humans.
2. CLDN1, referred to as CLD1, is an important component of tight junctions, which are crucial for maintaining the integrity and barrier function of epithelial cells.
3. The gene CLDN1 is responsible for the production of Claudin-1, a protein that plays a role in various cellular processes, including cell adhesion and signal transduction.
4. In addition to its role in cell adhesion, Claudin-1 is also implicated in regulating the permeability of tight junctions and the movement of ions and solutes across epithelial cell layers.
5. The protein CLDN1 is commonly referred to as Cldn1 and ILVASC. It is a key player in the organization and function of tight junctions, which are crucial for the maintenance of tissue homeostasis.
6. SEMP1, another name for Senescence-associated epithelial membrane protein 1, represents the association of Claudin-1 with cellular senescence, a state of irreversible cell cycle arrest associated with aging.
7. The expression of Claudin-1 is often altered in various diseases, including cancer, where it can affect tumor growth, invasion, and metastasis.
8. Understanding the role of CLDN1, or Claudin-1, is crucial in developing potential therapeutic strategies for diseases involving epithelial barriers, such as inflammatory bowel disease and cancer.
9. The study of Senescence-associated epithelial membrane protein 1, or Claudin-1, has provided insights into the intricate mechanisms governing cellular interactions and tissue homeostasis.
10. Further research on Claudin-1 and its functional properties may uncover novel therapeutic targets for diseases characterized by compromised epithelial barrier function.

Source

Rabbit

Specificity

The total Claudin 1 levels in the body can be detected using the Claudin 1 antibody, which specifically targets the endogenous Claudin 1 protein.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilution for Western blotting is between 1:500 and 1:3000, while for immunohistochemistry it is suggested to use a dilution between 1:50 and 1:200. When performing ELISA with peptide, the recommended dilution range is 1:20000-1:40000. These dilutions have been optimized to provide the best results for each application, so it is important to follow them carefully to achieve accurate and reliable data.

Immunogen

Analogous to human Claudin 1, a peptide synthesized in the laboratory has been developed. The synthesized peptide shares high similarities with the natural human protein and is a promising candidate for various therapeutic interventions. Through a series of molecular techniques, the peptide was designed to exhibit similar structural and functional characteristics to those found in the natural protein. This synthetic approach opens up exciting possibilities for the development of targeted therapeutics and drug discovery.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized in the purification of the antiserum through the process of peptide affinity chromatography. This method enabled the isolation of purified antiserum with high specificity towards the desired peptide.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended that the product be stored at a temperature of -20 °C for a period of one year. Avoid subjecting the product to repeated freeze/thaw cycles, as this may compromise its quality and efficacy. Proper storage can help maintain the stability and integrity of the product, making it more effective for its intended use. Therefore, it is important to follow these guidelines when storing the product for optimal results.

Storage buffer

This Rabbit IgG is stored in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it is advised to store it at a temperature of -20°C, and it can last for 12 months from the date of receipt.

Target

Background

Claudins are vital components of tight junction complexes that regulate the permeability of epithelia. They are major constituents, playing crucial roles in creating impermeable barriers or allowing the passage of ions and small molecules. Interactions between different claudin family members determine the overall permeability. CLDN1, specifically found in the epidermis, is essential for preventing the diffusion of small molecules through tight junctions and maintaining the skin's protective barrier function. It also contributes to water homeostasis, preventing excessive water loss through the skin. Interestingly, CLDN1 may indirectly influence the expression levels of other proteins to regulate water barrier formation in keratinocyte tight junctions. Overall, claudins, particularly CLDN1, are crucial for maintaining the integrity and functions of epithelial tissues, including the skin (PubMed:23407391).

Tissue specificity

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Celluar localization

Plasma Membrane;

UniPort

O95832