Phospho-LKB1 (Ser428) Rabbit Polyclonal Antibody#abs131078

abs131078-50ug

1-2 Weeks

201

abs131078-100ug

1-2 Weeks

301

Description

LKB1, a member of the CAMKL family, is a kinase that is widely expressed in various tissues. It exhibits tumor suppressor activity by phosphorylating AGS3 GPR domains, which in turn regulates the interaction between GPR-containing proteins and G-proteins. The highest levels of LKB1 expression are found in the testis and fetal liver. Mutations in LKB1 are associated with the development of Peutz-Jeghers cancer syndrome.

Other names

LKB1, also known as Liver kinase B1, is a protein involved in cellular processes such as polarization and renal carcinoma antigen NY-REN-19. It is a serine/threonine protein kinase that goes by various names including Polarization related protein LKB1 and Serine/threonine kinase 11. Additionally, it is referred to as Serine/threonine protein kinase XEEK1 and Stk11. LKB1 plays a crucial role in signaling pathways and is commonly associated with conditions like Peutz-Jeghers syndrome (PJS). In humans, it is coded by the STK11 gene.

Source

Rabbit

Specificity

The Antibody against Phospho-LKB1 (Ser428) specifically recognizes the phosphorylation of LKB1 at Serine 428. It is capable of detecting endogenous levels of this protein but only when it is in its phosphorylated state. To ensure accuracy and specificity, this antibody has been developed with high sensitivity and selectivity to phospho-LKB1 at Serine 428. This enables researchers to study the function and role of LKB1 under various physiological conditions and diseases with a high degree of confidence.

Predictive reaction species

Pig;Sheep;Dog;Horse;Bovine

Application

，：
（Western Blot）1:500-1:2000，（Immunohistochemistry）1:50-1:200，（Immunofluorescence/Immunocytochemistry）1:100-1:500，（Enzyme-linked immunosorbent assay， ELISA）（ELISA）1:20000-1:40000。

LKB1428。，。

Properties

1mg/ml

Purification

Through sequential chromatography on phospho- and non-phospho-peptide affinity columns, we were able to achieve the purification of the antibody from purified rabbit serum using affinity purification techniques. The resulting antibody product is highly purified and is based on the original serum sample.

Clonality

Polyclonal Antibody

To ensure preservation, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to freeze/thaw cycles repeatedly.

Storage buffer

This product contains Rabbit IgG in a buffer solution of phosphate and saline. The pH level is 7.4, and it also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that this product be stored at -20°C to maintain its stability and effectiveness. The product is stable for up to 12 months after the date of receipt.

Target

Background

 Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).

Tissue specificity

Ubiquitously expressed. Strongest expression in testis and fetal liver.

Celluar localization

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

Q15831