Phospho-c-Jun (Ser63) Rabbit Polyclonal Antibody#abs130717

abs130717-50ug

1-2 Weeks

201

abs130717-100ug

1-2 Weeks

301

Description

The potential transforming gene of avian sarcoma virus 17 is believed to code for a protein that bears a striking resemblance to the viral protein. This protein is known to directly interact with specific DNA sequences, thereby playing a pivotal role in the regulation of gene expression. By rearranging the provided content, a highly similar version can be generated.

Other names

AP1, also known as activator protein 1, is a transcription factor that plays a crucial role in regulating gene expression. It is comprised of various protein subunits, including cJun and Jun proto oncogene, which bind to enhancer elements and promote transcriptional activity. In particular, cJun is a key component of AP1 and is involved in a variety of cellular processes such as cell growth, differentiation, and apoptosis. The oncogene JUN is also a member of the AP1 complex and is implicated in tumorigenesis. Overall, AP1 is an important regulator of gene expression and has a wide range of functions in various biological processes.

Source

Rabbit

Specificity

The Phospho-c-Jun (Ser63) Antibody is designed to specifically recognize endogenous levels of c-Jun protein that have undergone phosphorylation at Serine 63. This antibody is not capable of detecting unphosphorylated c-Jun protein, or c-Jun protein that has been modified at other sites. By targeting this specific form of c-Jun, researchers can gain insights into the signaling pathways that regulate this critical transcription factor in various cellular processes. The antibody can be used in a variety of applications, including Western blotting and immunohistochemistry. Overall, this antibody is an essential tool for investigating the complex network of molecular events that govern gene expression and cellular behavior.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

The recommended dilution for Western blot is 1:500 to 1:2000, for immunohistochemistry is 1:50 to 1:1000, and for ELISA (peptide) is 1:20000 to 1:40000. Please note that this is just a rearranged version of the original text information and does not follow the dialogue format of generated content.

A peptide has been created by synthesizing human c-Jun near the phosphorylation site of Serine 63. To generate an alternate version of this information, I will present it in a completely different format.

Properties

1mg/ml

Purification

Sequential chromatography on phospho- and non-phospho-peptide affinity columns was used to purify the antibody from rabbit serum. The resulting product is highly pure, and has been shown to be effective in many applications. The affinity purification process ensures that the antibody specifically binds to the target protein, providing high sensitivity and accuracy in detection. With its high specificity and purity, this antibody is an excellent tool for the study of protein function and regulation.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to avoid exposing the product to repeated freeze-thaw cycles as it may affect its efficacy. Please make sure to follow the recommended storage conditions to ensure the product's integrity and potency.

Storage buffer

This product consists of Rabbit IgG dissolved in a solution of phosphate buffered saline at pH 7.4, with 150mM of NaCl, 0.02% of sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C for optimal preservation. The product is stable up to 12 months from the date of receipt. Please take note of the storage instructions to ensure the longevity of the product.

Target

Background

 Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Celluar localization

Cytosol;Nucleus;

P05412