Phospho-PKCB (Ser661) Rabbit Polyclonal Antibody#abs130724

abs130724-50ug

1-2 Weeks

201

abs130724-100ug

1-2 Weeks

301

Description

The protein Pim1 is known to be involved in signal transduction within blood cells. It has been hypothesized that Pim1 is able to phosphorylate HP1 gamma/CBX3, which may lead to structural changes or the silencing of chromatin. This phosphorylation may have an impact on the regulation of gene expression within cells.

Other names

Protein kinase C beta, also known as PKC beta, PKC-B, PKCB, or Prkcb, is a crucial enzyme that plays a vital role in various cellular functions. There are two isoforms of PRKCB, namely PRKCB I and PRKCB II, which are encoded by PRKCB1 and PRKCB2 genes, respectively. PRKCB belongs to the family of serine/threonine kinases and is involved in signal transduction pathways that regulate cell growth, differentiation, and proliferation.
Protein kinase C beta type has been extensively studied for its involvement in various diseases such as cancer, diabetes, and cardiovascular disorders. Studies have shown that abnormal expression or activity of PRKCB can lead to the development of these diseases. Researchers are currently investigating PRKCB as a potential therapeutic target for the treatment of these conditions.
In summary, PKC beta is a crucial enzyme that regulates various cellular functions, and its dysregulation is associated with the development of several diseases. Further research is needed to fully understand the role of PRKCB in disease pathogenesis and to develop effective therapeutic strategies targeting PKC beta.

Source

Rabbit

Specificity

The antibody Phospho-PKCB (Ser661) is designed to specifically identify and detect the presence of phosphorylated PKCB at Serine 661 in endogenous levels. This means that it is able to recognize PKCB only when it is phosphorylated at this specific serine residue.

Predictive reaction species

Sheep;Bovine;Rabbit

Application

The recommended dilutions for using the antibody in various applications are as follows: Western blotting should be done with a dilution range of 1:500 to 1:2000, while IF/ICC should be done at a dilution range of 1:100 to 1:500. For ELISA using a peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to ensure that appropriate dilutions are used to achieve optimal results in experiments.

A peptide synthesized from the phosphorylation site of Serine 661 in human PKCB is desired. Please produce an alternative text while ensuring it is derived from the original information given, disregarding the approach of content generation used by ChapGPT.

Properties

1mg/ml

Purification

，。

Clonality

Polyclonal Antibody

To ensure the integrity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The rabbit IgG solution is formulated with phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol for enhanced stability. The solution should be stored at a temperature of -20 °C and is guaranteed to remain stable for up to 12 months from the date of receipt. This product is perfect for those who require a reliable source of rabbit IgG for their research or laboratory needs. Order today and enjoy high-quality IgG that is designed to provide accurate and dependable results every time.

Target

Background

This protein kinase, which is dependent on calcium, phospholipids, and diacylglycerol (DAG), plays important roles in various cellular processes. It regulates B-cell receptor signal transduction, apoptosis induced by oxidative stress, transcription regulation of the androgen receptor, insulin signaling, and proliferation of endothelial cells. One of its key functions is to activate the canonical NF-kappa-B pathway through direct phosphorylation of CARD11/CARMA1. This leads to the recruitment of the BCL10-MALT1 complex and MAP3K7/TAK1, which activate the IKK complex, resulting in NFKB1 nuclear translocation and activation. This protein kinase also negatively regulates BTK function by directly phosphorylating BTK at Ser-180. It is involved in regulating apoptosis following oxidative damage and may function downstream of IRS1 in insulin signaling to mediate insulin-dependent DNA synthesis. Moreover, it plays a role in regulating glucose transport in adipocytes and inhibiting the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. In pancreatic beta-cells, it may inhibit insulin gene transcription via MYC regulation. In endothelial cells, activation of PRKCB leads to increased phosphorylation of RB1, VEGFA-induced cell proliferation, and inhibition of PI3K/AKT-dependent nitric oxide synthase regulation by insulin, causing endothelial dysfunction. Additionally, it phosphorylates ATF2, promoting cooperation between ATF2 and JUN and activating transcription. Finally, this protein kinase is involved in triglyceride homeostasis.

Celluar localization

Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P05771