Rabbit Anti-MAPK3 Polyclonal Antibody #abs130419

abs130419-50ug

1-2 Weeks

201

abs130419-100ug

1-2 Weeks

301

Description

The Ser/Thr protein kinase family encompasses this particular gene, encoding a protein kinase that acts as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. Serving as integration points for various biochemical signals, MAP kinases, or extracellular signal-regulated kinases (ERKs), play a crucial role. When cells are stimulated by growth factors or stress, this kinase becomes activated. In vitro studies have proven that ERK, p38 MAP kinase, and Jun N-terminal kinase can all phosphorylate and activate this specific kinase. This indicates that it acts as an integrating component in signaling pathways related to both mitogen and stress responses. Notably, this kinase interacts with, phosphorylates, and suppresses the activity of E47, a transcription factor with a basic helix-loop-helix structure. E47 is involved in regulating the expression of tissue-specific genes and promoting cell differentiation.

Other names

3pK, also known as anti MAPKAPK3 or Chromosome 3p kinase, is an enzyme classified under EC 2.7.11.1. It is commonly referred to as MAP kinase-activated protein kinase 3 or MAPKAPK3. This protein plays a crucial role in cellular signaling pathways. MAPKAPK3 is encoded by the MAPK3_HUMAN gene and is also known as MAPKAP, MAPKAP kinase 3, MAPKAP-K3, MAPKAP3, or MAPKAPK-3.
Another name for MAPKAPK3 is Mitogen-activated protein kinase-activated protein kinase 3. This name highlights its activation by MAP kinases, which are involved in crucial cellular processes. Moreover, it is important to note that MAPKAPK3 is a target of inhibitors such as MK-3.
In summary, 3pK (anti MAPKAPK3 or Chromosome 3p kinase) is an important enzyme involved in cellular signaling pathways. Its alternative names include MAP kinase-activated protein kinase 3, MAPKAP, MAPKAP kinase 3, MAPKAP-K3, MAPKAP3, MAPKAPK-3, and Mitogen-activated protein kinase-activated protein kinase 3. Additionally, it can be inhibited by MK-3.

Source

Rabbit

Specificity

MAPK3 Antibody detects endogenous levels of MAPK3.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Chicken;Rabbit;Horse;Pig;

Application

The recommended dilution for the use of this antibody in Western blot (WB) is between 1:500 to 1:2000, for Immunohistochemistry (IHC) the dilution range is between 1:50 to 1:200, for In-Cell Western/Immunofluorescence (IF/ICC) the dilution range is between 1:100 to 1:500, and for ELISA (peptide) the dilution range is between 1:20000 to 1:40000. Follow the recommended dilution guidelines to achieve optimal results with this antibody.

Immunogen

A synthesized peptide derived from human MAPK3.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out via peptide affinity chromatography. To ensure a highly purified antiserum, this method was employed for its efficient separation of the target protein.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at -20 °C for a duration of one year. It is crucial to avoid subjecting it to repeated freeze/thaw cycles as this may compromise its quality.

Storage buffer

This product is Rabbit IgG suspended in a solution of phosphate buffered saline at a pH of 7.4, with 150mM NaCl and 0.02% sodium azide. It is also combined with 50% glycerol to help preserve the integrity of the sample during storage. To maintain its quality, it is recommended that the product be stored at a temperature of -20°C. It is safe to use for a period of 12 months from the date of receipt.

Target

Background

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

Tissue specificity

Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. Expressed in the retinal pigment epithelium (PubMed:26744326).

Posttranslational modification

Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 (By similarity).

Celluar localization

Cytosol;Nucleus;

UniPort

Q16644