Phospho-FKHRL1 (Ser253) Rabbit Polyclonal Antibody#abs130648

abs130648-50ug

1-2 Weeks

201

abs130648-100ug

1-2 Weeks

301

Description

This gene is a member of the forkhead family of transcription factors, known for their unique forkhead domain. Its main role is believed to be initiating apoptosis, a process that involves activating the expression of necessary genes for cell death.

Other names

FOXO3A protein, also known as FKHRL1 or Forkhead box O3, is encoded by the AF6q21 gene. It is a homolog of the Forkhead Drosophila protein and has been associated with rhabdomyosarcoma. FOXO3A is involved in the regulation of various biological processes and is known to play a crucial role in cellular response to stress, apoptosis, and aging. It is also implicated in the development and progression of certain cancers. The gene is expressed in multiple tissues and has different isoforms, including FOXO3 and FOXO3A. MGC12739 and MGC31925 are alternative names for FOXO3A protein.

Source

Rabbit

Specificity

The Antibody Phospho-FKHRL1 (Ser253) is designed to specifically target and identify the presence of phosphorylated FKHRL1 at Serine 253. Its purpose is to detect natural levels of FKHRL1 when it is modified by phosphorylation at this particular serine residue. By recognizing and binding to the phosphorylated protein, this antibody enables researchers to study and analyze the cellular processes and functions associated with FKHRL1 in its phosphorylated state.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

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For Western blotting, dilute the primary antibody at a range of 1:500 to 1:2000. In the case of immunohistochemistry, the primary antibody should be diluted from 1:50 to 1:1000. For enzyme-linked immunosorbent assay or ELISA using a peptide, we recommend a dilution range of 1:20000 to 1:40000. These are the suggested dilution ranges for optimal results.

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"The phosphorylation site of Serine 253 in human FKHRL1 has been utilized to synthesize a novel peptide. This synthesized peptide, derived from FKHRL1, shows promising potential for further research and development. By focusing on the phosphorylation site, we aim to uncover new insights into the functional relevance of FKHRL1 in cellular processes. The synthesized peptide offers a unique opportunity to study its interactions, signaling pathways, and potential therapeutic applications. This innovative approach opens up new avenues for understanding the role of FKHRL1 and its phosphorylation site in disease mechanisms and molecular pathways."

Properties

1mg/ml

Purification

Through a process of affinity purification involving sequential chromatography on phospho- and non-phospho-peptide affinity columns, a high-quality antibody was successfully isolated from purified rabbit serum. The resulting antibody possesses a high degree of similarity to the original serum and is capable of efficiently detecting its target antigen. This development provides a valuable resource for biomedical research and has the potential to contribute to the development of new therapies and diagnostic tools.

Clonality

Polyclonal Antibody

-20°C。/。

Storage buffer

The recommended storage conditions for Rabbit IgG are at -20°C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures the stability of the IgG for a period of 12 months from the date of receipt. Please note that it is important to follow these storage guidelines to maintain the quality and efficacy of the product.

Target

Background

 Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Tissue specificity

Ubiquitous.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

O43524