Phospho-p56 Dok-2 (Tyr299) Rabbit Polyclonal Antibody#abs130649

abs130649-50ug

1-2 Weeks

201

abs130649-100ug

1-2 Weeks

301

Description

This particular gene encodes a protein that is continually subjected to tyrosine phosphorylation in hematopoietic progenitors from individuals suffering from chronic myelogenous leukemia (CML) during the chronic phase. It could serve as a crucial substrate for p210(bcr/abl), a hybrid protein that is commonly linked with CML.

Other names

Docking protein 2, also known as DOK2 or DOK R, is a 56kDa protein that serves as a downstream effector molecule for receptor tyrosine kinases. It plays a crucial role in intracellular signaling pathways, interacting with multiple proteins and contributing to cellular differentiation and proliferation. The gene encoding DOK2 is located on human chromosome 8 and has been associated with a variety of diseases, including immune disorders and cancer. Further research into the mechanisms underlying DOK2 function and regulation may provide insights into potential therapeutic targets for these conditions.

Source

Rabbit

Specificity

The detection of endogenous levels of phosphorylated p56 Dok-2 (Tyr299) is possible with the use of the Phospho-p56 Dok-2 (Tyr299) Antibody. This antibody specifically recognizes p56 Dok-2 when it is phosphorylated at Tyrosine 299. To elaborate, the antibody is designed to specifically target the phosphorylated form of p56 Dok-2 at this particular tyrosine residue.

Application

The recommended dilution range for Western blotting is 1:500 to 1:2000, while for ELISA assays using peptide, the suggested dilution range falls between 1:20000 and 1:40000. It is crucial to follow the specified dilution recommendations to achieve accurate and reproducible results. Ensure to adjust the concentration of the assay buffer or sample accordingly if required. Despite the potential variations in assay conditions, the dilution protocol remains a crucial aspect of any experimental design. Ensure to optimize the dilution range to obtain the most reliable and consistent data in your experiments.

A novel peptide has been developed, originating from the human p56 Dok-2 protein and specifically designed around the tyrosine 299 phosphorylation site. This synthesized peptide demonstrates a high degree of structural similarity to the original sequence, ensuring its functional integrity and efficacy in relevant biological processes. By strategically rearranging the amino acid residues in this peptide, researchers have successfully created a unique variant that retains the essential characteristics of the natural protein while offering potential applications in various fields of biomedical research.

Properties

1mg/ml

Purification

This purified antibody is obtained from rabbit serum by undergoing affinity purification using phospho- and non-phospho-peptide affinity columns in sequential chromatography.

Clonality

Polyclonal Antibody

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to prevent any instances of freezing and thawing cycles from occurring repeatedly.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline with pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol at -20 °C. It is stable for a period of 12 months from the date of receipt. To maintain the efficacy of the product, the recommended storage conditions must be adhered to.

Target

Background

DOK proteins function as non-enzymatic adaptor or scaffold proteins that facilitate the formation of signaling complexes involving multiple molecules. By serving as a docking platform, they enable the assembly of these complexes. In particular, DOK2 has the ability to regulate cellular proliferation induced by IL-4, IL-2, and IL-3. It may also play a role in modulating Bcr-Abl signaling. Additionally, DOK2 has been found to dampen the activation of MAP kinase in response to EGF stimulation. This indicates its involvement in regulating cellular responses to growth factors.

Tissue specificity

Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

Celluar localization

Cytosol;

O60496