Phospho-JAK2 (Tyr1007) Rabbit Polyclonal Antibody#abs130650

abs130602-50ug

1-2 Weeks

201

abs130602-100ug

1-2 Weeks

301

Description

This protein tyrosine kinase plays a crucial role in a particular subset of cytokine receptor signaling pathways and is known to associate with the prolactin receptor in a constant manner. It is indispensable for eliciting responses to gamma interferon. By rearranging the provided text, a highly similar content can be generated to convey the same information. However, it is important to note that the approach of generating significantly distinct content from the language model should be followed for a more divergent conversation.

Other names

Janus Activating Kinase 2, also known as JAK 2 or JAK2, is a protein tyrosine kinase. It plays a crucial role in various cellular processes and signaling pathways. The human JAK2 gene, labeled JAK2_HUMAN, encodes this kinase. JAK2 is involved in the activation of Janus kinases and subsequently stimulates cell growth and differentiation.
Another name for JAK2 is JTK 10 or JTK10. It is also referred to as OTTHUMP00000043260 and THCYT3. JAK2 is classified as a tyrosine protein kinase due to its ability to phosphorylate tyrosine residues on target proteins. This phosphorylation event triggers downstream signaling cascades and regulates gene expression.
Understanding the function of JAK2 is crucial in various fields of research, including cancer biology and immunology. Many studies aim to unravel the intricate mechanisms by which JAK2 is regulated and how it influences cellular processes. By gaining a deeper insight into JAK2, scientists hope to develop targeted therapies for diseases associated with dysregulated JAK2 activity.
In summary, JAK2 is a vital protein tyrosine kinase that acts as a signaling molecule in several biological pathways. Its role in cell growth and differentiation makes it an attractive target for therapeutic interventions. The extensive research conducted on JAK2 enhances our understanding of its functions and offers opportunities for developing novel treatments.

Source

Rabbit

Specificity

The Phospho-JAK2 (Tyr1007) Antibody is designed to specifically recognize and bind to JAK2 protein only when it is phosphorylated at Tyrosine 1007. This antibody allows for the detection of endogenous levels of phosphorylated JAK2, indicating its activation state in various biological samples. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways regulated by JAK2 and its role in cellular processes.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

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The dilution ratios for various laboratory techniques are as follows:
- Western Blotting (WB): 1:1000
- Immunohistochemistry (IHC): 1:50-1:200
- Immunofluorescence/Immunocytochemistry (IF/ICC): 1:100-1:500
- Enzyme-Linked Immunosorbent Assay (ELISA) using peptide: 1:20000-1:40000
These dilution ratios are standard for these techniques and are used to determine the optimal concentration of antibodies or reagents necessary for accurate results. It is important to dilute the primary antibodies appropriately to ensure specific and reliable detection of the target protein or antigen.

Human JAK2-derived peptide, specifically designed around the phosphorylation site of Tyrosine 1007, has been synthesized. The synthesized peptide closely resembles the original human JAK2 sequence and includes the crucial tyrosine phosphorylation site at position 1007.

Properties

1mg/ml

Purification

，。，。

Clonality

Polyclonal Antibody

Ensure that the storage temperature is maintained at -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing.

Storage buffer

This product contains Rabbit IgG in a buffered saline solution with a pH of 7.4, 150 mM NaCl, and 0.02% sodium azide, combined with 50% glycerol. It should be stored at a temperature of -20°C and has a shelf life of 12 months from the date of receipt.
To ensure maximum stability and efficacy, it is recommended that this product be stored in a cool, dry place and protected from direct sunlight or extreme temperatures. Prior to use, it should be thawed slowly at room temperature and gently mixed until fully dissolved.
This Rabbit IgG solution is ideal for various applications in immunology research, including western blotting, ELISA, and immunohistochemistry. Its highly specific affinity for a wide range of antigens makes it a valuable tool for the detection and quantification of target proteins in biological samples.
Overall, this product provides a reliable and effective solution for immunology research applications, backed by rigorous quality testing and a proven track record of success in the field.

Target

Background

JAK2 is a non-receptor tyrosine kinase that plays a critical role in various cellular processes such as growth, differentiation, and histone modifications. It is also involved in mediating intrinsic and adaptive immunity signaling. JAK2 is located in the cytoplasm and forms a complex with type I receptors such as growth hormone, prolactin, leptin, erythropoietin, and thrombopoietin. It also interacts with type II receptors such as IFN-alpha, IFN-beta, IFN-gamma, and interleukins.
Once JAK2 is activated following ligand-binding to cell surface receptors, it then phosphorylates specific tyrosine residues on the receptor’s cytoplasmic tail. This creates a docking site for STATs proteins, which are then recruited to the receptor and phosphorylated by JAK2. The phosphorylated STATs proteins form a homodimer or heterodimer, which then translocates to the nucleus and triggers gene transcription.
For instance, when stimulated with erythropoietin (EPO), JAK2 autophosphorylates and interacts with erythropoietin receptor (EPOR), leading to STAT5A or STAT5B recruitment and activation. The activated STAT5A or STAT5B dimer translocates into the nucleus and promotes the expression of genes that modulates erythropoiesis.
JAK2 also plays a crucial role in cell cycle progression and cytokine-driven FOS transcription activation. In the nucleus, it regulates chromatin function by specifically phosphorylating histone H3 at tyrosine 41 (H3Y41ph), which in turn promotes the exclusion of CBX5 (HP1 alpha) from chromatin.

Tissue specificity

Ubiquitously expressed throughout most tissues.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Nucleus;Plasma Membrane;

O60674