Phospho-JAK2 (Tyr931) Rabbit Polyclonal Antibody#abs130652

abs130652-50ug

1-2 Weeks

201

abs130652-100ug

1-2 Weeks

301

Description

A vital protein tyrosine kinase that participates in particular cytokine receptor signaling pathways has been identified. It is constantly associated with the prolactin receptor and plays a crucial role in the response to gamma interferon.

Other names

Janus Activating Kinase 2, also known as JAK 2 or JAK2, is a protein tyrosine kinase. It plays a crucial role in cellular signaling pathways. JAK2_HUMAN is the human form of this kinase. It is involved in the activation of STAT proteins, which are transcription factors that regulate gene expression. JTK 10 and JTK10 are alternative names for JAK2. This kinase is also referred to as kinase Jak2. Additionally, it is encoded by the gene OTTHUMP00000043260. JAK2 is classified as a tyrosine protein kinase as it phosphorylates tyrosine residues in target proteins. Its function is essential for various physiological processes including hematopoiesis and immune response.

Source

Rabbit

Specificity

The endogenous levels of JAK2 can be accurately detected by the Phospho-JAK2 (Tyr931) Antibody specifically when it is phosphorylated at Tyrosine 931. It is essential to note that this antibody is highly specific and detects only the phosphorylated form of JAK2 at this particular tyrosine residue.

Application

For Western Blot analysis, the recommended dilution range is 1:500 to 1:2000. When performing Immunohistochemistry, we suggest using a dilution range of 1:50 to 1:200. If using Immunofluorescence or Immunocytochemistry, a dilution of between 1:100 to 1:500 is advised. And, for ELISA using peptide, we recommend diluting at a range of 1:20000 to 1:40000. It is important to ensure that the correct dilution is used to achieve accurate and reliable results in your experiments. Please follow the recommended guidelines closely.

Please generate a rearranged and highly similar content based on the original text information, avoiding dialogue-style content generation and ensuring that the text is distinct from the way ChapGPT would generate it.
An engineered peptide has been developed using a segment of the human JAK2 protein centered around the phosphorylation site of Tyrosine 931. This synthesized peptide closely mimics the original structure and function of the JAK2 protein, specifically targeting its phosphorylation site. The design ensures a high degree of similarity to the natural JAK2 sequence, facilitating its use in research and potential therapeutic applications. By effectively mimicking the region surrounding Tyrosine 931, this peptide offers valuable insights into the regulatory mechanisms of JAK2 and its role in cellular signaling pathways.

Properties

1mg/ml

Purification

The purified rabbit serum was used to isolate the antibody via a process of affinity purification using both phospho- and non-phospho-peptide affinity columns in sequential chromatography. A highly similar content could be generated by stating that the antibody was obtained through a process involving sequential chromatography on affinity columns that were specifically designed for capturing non-phospho- and phospho-peptides in purified rabbit serum. This method of affinity purification allowed for the isolation of the antibody from the serum.

Clonality

Polyclonal Antibody

To ensure the quality of our product, it is recommended that you store it at a temperature of -20 °C for a period of one year. It's important to note that repetitive freeze and thaw cycles should be avoided, as this may affect the potency of the product. So, be sure to carefully follow the storage instructions to ensure the best possible outcome.

Storage buffer

The provided information has been rearranged to generate highly similar content:
Store Rabbit IgG in phosphate-buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol at -20 °C. It remains stable for 12 months from the date of receipt.

Target

Background

JAK2 is a non-receptor tyrosine kinase that plays a crucial role in various cellular processes such as cell growth, development, differentiation, and histone modifications. It is involved in both innate and adaptive immunity, mediating essential signaling events. In the cytoplasm, JAK2 interacts with type I receptors like growth hormone, prolactin, leptin, erythropoietin, thrombopoietin, as well as type II receptors including interferons and interleukins.
Upon ligand binding to cell surface receptors, JAK2 phosphorylates specific tyrosine residues on the receptor's cytoplasmic tails, creating docking sites for STAT proteins. Subsequently, the recruited STAT proteins are phosphorylated by JAK2. Once phosphorylated, STAT proteins form homodimers or heterodimers and translocate to the nucleus to initiate gene transcription.
For example, in erythropoiesis, JAK2 is activated and associates with the erythropoietin receptor (EPOR) upon stimulation with erythropoietin (EPO). This leads to the phosphorylation and activation of STAT5, which then translocates to the nucleus and promotes the transcription of genes involved in erythropoiesis.
JAK2 also plays a role in cell cycle regulation by phosphorylating CDKN1B. It cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription.
In the nucleus, JAK2 has a significant impact on chromatin by specifically phosphorylating 'Tyr-41' of histone H3 (H3Y41ph). This phosphorylation serves as a specific tag that promotes the exclusion of CBX5 (HP1 alpha) from chromatin, thereby modulating chromatin structure and function. Overall, JAK2 is a multifunctional kinase with critical implications in various physiological and pathological processes.

Tissue specificity

Ubiquitously expressed throughout most tissues.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Nucleus;Plasma Membrane;

O60674