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Rabbit Anti-COX IV Polyclonal Antibody#abs131570

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Description

Catalog-specification	Delivery time	USD price
abs131570-100ug	1-2 Weeks	301.0
abs131570-50ug	1-2 Weeks	201.0

Overview
catalog	abs131570
Description	If you're looking for a reliable loading control for mitochondrial proteins, we recommend using an antibody against COX IV. This antibody can effectively monitor protein loading as COX IV is typically expressed at a consistent high level. However, it's worth noting that some other proteins may also run at the 16kD size of COX IV which could cause issues. In this case, we suggest using our VDAC1 / Porin antibody as an alternative mitochondrial loading control for proteins of this size. By doing so, you can ensure more accurate protein quantification and ultimately increase the validity of your experimental results.
Other names	COX4, also known as cytochrome c oxidase subunit IV, is a crucial component of the respiratory chain complex IV. It is responsible for the final step in the electron transport chain, where it transfers electrons from cytochrome c to molecular oxygen, resulting in the production of water. This process plays a vital role in energy production within the mitochondria. There are two isoforms of COX4 identified in humans, namely COX IV-1 and COX IV-2. Both isoforms are localized in the mitochondria and are encoded by different genes, COX4I1 and COX4I2, respectively. COX IV-1 is the predominant isoform found in most tissues, while COX IV-2 is specifically expressed in lung tissue. Mutations or dysregulation in COX4 can lead to various mitochondrial disorders, including Leigh syndrome, cardioencephalomyopathy, and encephalopathy. These conditions are characterized by impaired energy production, resulting in tissue damage and dysfunction. Therefore, understanding the structure and function of COX4 is crucial for elucidating the underlying mechanisms of these disorders and developing potential therapeutic interventions.
Source	Rabbit
Specificity	The COX IV antibody is capable of identifying the complete amount of endogenous COX IV. To convey a similar message in a different way, the antibody specifically targets and detects the total levels of COX IV present in the sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	COX IV
Application	The recommended dilutions for using this antibody in various applications are as follows: for Western Blotting, dilute the antibody at a range of 1:500-1:2000; for Immunohistochemistry, dilute at a range of 1:50-1:200; for Immunofluorescence, dilute at a range of 1:100-1:500; and for ELISA (peptide), dilute the antibody at a range of 1:20000-1:40000. These dilution ranges will ensure optimal results and specificity of the antibody for the intended application. It is important to note that the appropriate dilution may vary based on the sample type and experimental conditions, and optimization may be necessary for each individual experiment. Overall, this antibody is a useful tool for researchers studying the target protein in various applications.
Immunogen	A synthesized peptide derived from human COX IV.
MW	17 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Tissue specificity	Ubiquitous.
Celluar localization	Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	P13073