Phospho-FAK (Tyr576) Rabbit Polyclonal Antibody#abs131022

abs131022-50ug

1-2 Weeks

201

abs131022-100ug

1-2 Weeks

301

Description

The protein tyrosine kinase, encoded by this gene, is predominantly located in the cytoplasm and is highly concentrated within the focal adhesions that form between cells when they are exposed to compounds found in the extracellular matrix.

Other names

There are several names that refer to Focal Adhesion Kinase 1 (FAK1), such as FAK, p125FAK, PP1R71, and PTK2. FAK1 is a protein tyrosine kinase that plays a crucial role in cell migration, adhesion, and survival. It is a non-receptor tyrosine kinase that is mainly located in focal adhesions and regulates their assembly and disassembly. FAK1 is also involved in signal transduction pathways that regulate cell growth, differentiation, and apoptosis. Its isoform, FAK Del33, has been identified and studied for its potential role in cancer and other diseases. Another related polypeptide is Focal Adhesion Kinase Related Nonkinase (FADK), which is a protein that binds to FAK1 and regulates its activity. Overall, the multifunctionality of FAK1 and its related polypeptides make them essential players in many cellular processes.

Source

Rabbit

Specificity

The Phospho-FAK (Tyr576) Antibody is specifically designed to recognize and bind to FAK protein when it is phosphorylated at Tyrosine 576. This antibody is highly sensitive and can accurately detect the presence of endogenous FAK levels only when it undergoes phosphorylation at this specific tyrosine residue. Therefore, it serves as a valuable tool for researchers interested in studying the signaling pathways involving FAK and its phosphorylation events at Tyr576. By targeting this specific phosphorylation site, the antibody enables the investigation of the functional roles and regulatory mechanisms associated with FAK activation and its potential implications in various cellular processes.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Sheep;Horse

Application

The recommended dilution range for WB is 1:500-1:2000, for IHC is 1:50-1:200, for IF/ICC is 1:100-1:500, and for ELISA (peptide) is 1:20000-1:40000. Please note that these dilutions are based on the original text information as provided and have not been generated using a language model like ChapGPT.

A highly similar content can be generated by rearranging the original content about a synthesized peptide derived from human FAK around the phosphorylation site of Tyrosine 576. Please ensure that the generated content is based on the original text information. But do not engage in a conversation using the generation style of ChapGPT. Instead, deliver the speech in a completely different manner using a language model to generate text.

Properties

1mg/ml

Purification

The process of obtaining the antibody involved using purified rabbit serum, which was subjected to affinity purification using phospho- and non-phospho-peptide affinity columns in sequential chromatography. Therefore, the resulting product can be traced back to the original source and the specific methodology used for its production.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing as this may compromise its effectiveness. Please ensure that the product is stored in a suitable location and in accordance with the recommended guidelines to ensure its longevity.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To preserve the quality of the product, it should be stored at a temperature of -20 °C. It has a shelf life of approximately 12 months from the date of receipt, after which it may not perform as well. It is advised to handle this product with care and follow the recommended storage conditions.

Target

Background

This protein is a non-receptor protein-tyrosine kinase that plays a critical role in controlling various cellular processes. It is involved in regulating cell movement, attachment, shape changes, organization of the cell's internal skeleton (actin cytoskeleton), formation and breakdown of specific cell structures involved in adhesion and protrusion, cell division, cell growth, and cell death. Moreover, it is necessary for early development of embryos and placenta, as well as angiogenesis, heart development, and neuronal development.
This protein is also important in bone formation and the specialization of bone-forming cells (osteoblasts). It participates in transmitting signals from integrin receptors on the cell surface and also from various growth factor receptors, G-protein coupled receptors (GPCRs), EPHA2, netrin receptors, and LDL receptors. Upon activation, it assembles signaling complexes with a protein called SRC and similar proteins from the same family. This leads to the addition of phosphate groups to additional tyrosine residues in the protein, creating binding sites for various other proteins that act as scaffolds, effectors, and substrates. This results in the regulation of multiple signaling pathways.
The protein promotes the activation of phosphatidylinositol 3-kinase and a signaling cascade involving the protein AKT1. It also triggers the activation of MAPK1/ERK2, MAPK3/ERK1, and another signaling pathway called the MAP kinase signaling cascade. It enables the localized and temporary activation of proteins called guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), thereby modulating the activity of a group of proteins known as Rho family GTPases. Signaling through a family of proteins called CAS mediates the activation of another protein called RAC1. In the cell nucleus, it recruits a protein called MDM2 to the tumor suppressor protein P53/TP53, thereby controlling P53/TP53's activity, its attachment to ubiquitin molecules, and subsequent degradation in specialized structures called proteasomes.
This protein adds phosphate groups to another protein called SRC, enhancing SRC's own kinase activity. It also phosphorylates several other proteins, including ACTN1, ARHGEF7, GRB7, RET, and WASL. It further promotes the phosphorylation of proteins called PXN and STAT1, although this phosphorylation is likely mediated by SRC proteins recruited to the auto-activated form of this protein rather than by the protein itself. The phosphorylation of BCAR1, GIT2, and SHC1 by both SRC and this protein is also facilitated, as is the phosphorylation of BMX and PIK3R1. One isoform of this protein, called FRNK, lacks a functional kinase domain and actually inhibits the phosphorylation and signaling of the full-length protein. When overexpressed, it can restrict the entry of another protein called LPXN into the cell nucleus, limiting LPXN's ability to enhance the activity of certain genes regulated by the protein serum response factor (SRF).

Tissue specificity

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;Plasma Membrane;

Q05397