Phospho-PKC Zeta (Thr410) Rabbit Polyclonal Antibody#abs131029

abs131029-50ug

1-2 Weeks

201

abs131029-100ug

1-2 Weeks

301

Description

One of the key players in several cellular processes, including proliferation, differentiation and secretion, is protein kinase C (PKC) zeta. This serine/threonine kinase is part of the PKC family and its activity is critical for the regulation of numerous cellular functions. Through its various interactions, PKC zeta plays a crucial role in maintaining the proper balance of intracellular signaling pathways.

Other names

The 14-3-3 zeta isoform is also known as PKC Zeta, aPKCzeta, or nPKC-zeta. It is a protein kinase that plays an important role in signaling pathways. The gene encoding this protein is called PRKCZ. Some other names for this protein include PKC2, Protein kinase C zeta form, and Protein kinase C zeta type.
The 14-3-3 zeta isoform is involved in a variety of cellular processes, such as cell cycle regulation, signal transduction, apoptosis, and neuronal function. It interacts with many different proteins, including other kinases, transcription factors, and cytoskeletal proteins.
Studies have shown that the 14-3-3 zeta isoform may be involved in the development of certain diseases, such as cancer and Alzheimer's disease. Inhibition of this protein has been proposed as a potential therapeutic strategy for these and other diseases.
Overall, the 14-3-3 zeta isoform is an important protein with diverse functions. Its interactions with other proteins and involvement in signaling pathways make it a promising target for future research and therapeutic interventions.

Source

Rabbit

Specificity

The Phospho-PKC zeta (Thr410) Antibody is specifically designed to identify the presence of PKC zeta in a sample only if it is phosphorylated at Threonine 410. This antibody is capable of detecting endogenous levels of PKC zeta and is an essential tool for researchers examining signal transduction pathways in various biological systems. The data gathered using this antibody can provide valuable insights into the role of PKC zeta in various cellular processes and can help researchers develop targeted therapies for diseases that involve aberrant PKC zeta signaling.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Bovine;Pig;Horse;

Application

When it comes to using this particular antibody, there are some recommended dilutions to follow. For Western blotting, it is suggested to use a dilution of 1:500 to 1:2000. For immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. If you are looking to use this antibody in immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is advised. Finally, for ELISA with the peptide, a dilution of 1:20000 to 1:40000 is the recommended range. It's important to follow these guidelines to ensure the best results for your experiments.

A peptide that has been synthesized from the human PKC zeta protein, focusing on the Threonine 410 phosphorylation site, has been developed. This peptide is designed to closely resemble the original content and provide similar benefits for studying the protein's function.

Properties

1mg/ml

Purification

The purified rabbit serum was used to produce the antibody through a process of affinity purification involving sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To preserve the product quality, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to several freeze/thaw cycles.

Storage buffer

The recommended storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with a pH of 7.4. The solution contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol to maintain stability. It is important to note that this product has a shelf life of 12 months from the date of receipt.

Target

Background

This protein kinase is involved in various cellular processes, including NF-kappa-B activation, cell proliferation, cell polarity, inflammatory response, and maintenance of long-term potentiation. It functions in the PI3K pathway and MAP kinase cascade, independent of calcium and diacylglycerol. Following mitogenic stimuli, it activates the MAPK1/ERK2 signaling cascade without the need for RAF1 activation. It is also required for AKT3 activation and contributes to the activation of glucose transport in adipocytes. Furthermore, it stimulates neuronal differentiation, and is necessary and sufficient for LTP maintenance in hippocampal cells. In vein endothelial cells, it phosphorylates STK11, leading to inhibition of PI3K/Akt signaling and increased apoptosis. It can also relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit. In summary, this protein kinase plays a crucial role in various cellular processes and may be involved in the development of allergic airway inflammation.

Tissue specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Extracellular region or secreted;Nucleus;Plasma Membrane;

Q05513