Rabbit Anti-GLUT3 Polyclonal Antibody #abs130111

abs130111-50ug

1-2 Weeks

201

abs130111-100ug

1-2 Weeks

301

Description

GLUT3 is a type of integral membrane facilitative glucose transporter that belongs to the human equilibrative glucose transport protein family, which includes 13 members. Its expression on the surface is dependent on the presence of insulin. However, it is only found in the brain, testis, and skeletal muscle of humans. In slow-twitch fibers, it is mostly expressed in triads. It has been discovered that in mice and rats, it is only expressed in the brain.

Other names

The GLUT 3 protein, also known as glucose transporter type 3, is primarily found in the brain and is encoded by the FLJ90380 gene. This protein is responsible for facilitating the transport of glucose across the blood-brain barrier, which is essential for brain metabolism and function. Additionally, defects in GLUT 3 expression can lead to various neurological disorders. The human SLC2A3 gene encodes this protein, which belongs to the facilitated glucose transporter family 2 and contains 12 transmembrane domains. Overall, GLUT 3 plays a critical role in brain glucose uptake and metabolism, making it a crucial target for therapeutic interventions.

Source

Rabbit

Specificity

The detection of total GLUT3 at endogenous levels can be achieved using the GLUT3 antibody. To maintain consistency and accuracy, the content should be rearranged in a way that preserves the original text information. Please note that responses generated by GPT-3 can vary widely and may not always reflect the desired outcome.

Species Reactivity

Human

Application

Here are some variations of the original text:
- For western blotting, use a dilution range of 1:500 to 1:3000. Immunohistochemistry can be performed using a dilution range of 1:50 to 1:200. For immunofluorescence and immunocytochemistry assays, the recommended dilution range is 1:100 to 1:500. ELISA with peptide antigen requires a dilution range of 1:20000 to 1:40000.
- When using western blotting, we suggest diluting the primary antibody at a ratio of 1:500 to 1:3000. For immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. Immunofluorescence and immunocytochemistry assays should use a dilution range of 1:100 to 1:500. For ELISA with peptide antigen, the appropriate dilution range is 1:20000 to 1:40000.
- The optimal dilution range for western blotting is 1:500 to 1:3000, while immunohistochemistry requires a dilution range of 1:50 to 1:200. Immunofluorescence and immunocytochemistry assays should use the primary antibody at a dilution range of 1:100 to 1:500. ELISA with peptide antigen can be performed using a dilution range of 1:20000 to 1:40000.

Immunogen

A synthesized peptide derived from human GLUT3.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The resulting product was highly purified and contained a concentrated amount of antibodies.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality, it is advisable to store the item at a temperature of -20 °C for a duration of one year. It is important to refrain from subjecting it to multiple cycles of freezing and thawing to maintain its integrity.

Storage buffer

The rabbit IgG solution is composed of phosphate buffered saline with a pH of 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and can remain stable for up to 12 months from the date of receipt.

Target

Background

This facilitative glucose transporter is capable of facilitating the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). It plays a crucial role in transporting glucose, 2-deoxyglucose, galactose, mannose, xylose, and fucose, and possibly even dehydroascorbate (PubMed:9477959, PubMed:26176916). However, it does not participate in the transport of fructose (PubMed:9477959, PubMed:26176916). Generating a similar but distinct content, here is an alternative description:
With the ability to facilitate the transport of glucose and other monosaccharides across the cell membrane, this glucose transporter is highly versatile (PubMed:9477959, PubMed:26176916). Apart from glucose, it aids in the uptake of 2-deoxyglucose, galactose, mannose, xylose, fucose, and potentially dehydroascorbate as well (PubMed:9477959, PubMed:26176916). Notably, it does not serve as a mediator for fructose transport (PubMed:9477959, PubMed:26176916).

Tissue specificity

Highly expressed in brain. Expressed in many tissues.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

UniPort

P11169